不同防腐剂配方人工饲料对棉铃虫生长发育和繁殖的影响  

Effect of different artificial diet preservatives on the growth and reproduction of the cotton bollworm,Helicoverpa armigera

作　　者：屈荷丽[1] 段佳鑫 马思源 王少山[1] QU He-Li;DUAN Jia-Xin;MA Si-Yuan;WANG Shao-Shan(Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization,Xinjiang Uygur Autonomous Region,College of Agriculture,Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学农学院,新疆绿洲农业病虫害治理与植保资源利用重点实验室,石河子832000

出　　处：《应用昆虫学报》2024年第5期1048-1056,共9页Chinese Journal of Applied Entomology

基　　金：石河子大学农学院教改项目(nxyjb-2021-15)。

摘　　要：【目的】明确棉铃虫Helicoverpa armigera人工饲料中不同防腐剂对其生长发育和繁殖的影响,为实验室大规模饲养棉铃虫提供依据。【方法】将田间采集的棉铃虫成虫所产子代,分别用含有防腐剂0.16%山梨酸(饲料1)、0.16%山梨酸和0.09%甲醛(饲料2)、0.16%山梨酸和0.25%尼泊金甲酯(饲料3)的人工饲料连续饲养3代,测定不同世代棉铃虫的幼虫发育历期、蛹期、蛹重、化蛹率、成虫寿命和产卵量等发育指标。【结果】3种不同防腐剂配方的人工饲料对棉铃虫幼虫发育历期、化蛹率和蛹重影响显著,其中F_(1)代取食饲料2的幼虫发育历期为14.50d,显著短于饲喂饲料3(15.31d)(F_(2.337)=4.354,P<0.05),F_(2)、F_(3)代不同饲料间幼虫发育历期差异均不显著(P>0.05);饲料2饲养的3个世代棉铃虫幼虫化蛹率分别为83.33%、82.67%、90.67%,均显著高于饲料1和饲料3(F_(1)代:F_(2.6)=40.923,P<0.05,F_(2)代:F_(2.6)=6.454,P<0.05,F_(3)代:F_(2.6)=66.20,P<0.05);在F_(2)、F_(3)代,棉铃虫取食不同饲料对雌蛹重影响显著(F_(2)代:F_(2.163)=13.146,P<0.05,F_(3)代:F_(2.163)=7.207,P<0.05),而对羽化率、产卵量和子代卵孵化率等无显著影响(P>0.05)。【结论】饲料2使用山梨酸和甲醛作为人工饲料防腐剂,能显著降低棉铃虫幼虫死亡率,获得成虫数量最多,且成虫繁殖力高,更适合棉铃虫的大量饲养。[Aim]To determine the effects of different artificial diet preservatives on the growth,development and reproduction of the cotton bollworm,Helicoverpa armigera,in order to improve the captive propagation of this species.[Methods]Offspring of H.armigera were collected in the field and randomly assigned to one of three treatment groups(diet 1,diet 2 and diet 3),which were fed artificial diets with different preservatives for three generations.The preservative in diet 1 was sorbic acid,that in diet 2 was sorbic acid and formaldehyde and that in diet 3 was sorbic acid and methylparaben.Developmental indices of larvae in each treatment group,such as developmental duration,pupation rate,pupal weight and egg production,were measured and compared.[Results]The different preservatives had a significant effect on developmental duration,pupation rate and pupal weight,but no significant effect in female fecundity or the hatching rate of eggs laid by females raised on each diet.After 3 generations,larvae raised on diet 2 had a significantly higher pupation rate than those raised on diet 1 or diet 3,and the highest female fecundity.[Conclusion]Using a combination of sorbic acid and formaldehyde as the preservative of an artificial diet for H.armigera can significantly reduce larval mortality,increase the number of adults produced and adult female fecundity.

关 键 词：棉铃虫 防腐剂 人工饲料 发育历期 化蛹率 繁殖力 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治]