多黏类芽孢杆菌Paenibacillus polymyxa ZJ-9芽孢萌发条件与过程的研究  

Studies on spore germination conditions and process of Paenibacillus polymyxa ZJ-9

作　　者：黎槟瑞 张忆萱 高健 徐虹[1] 李莎[1] LI Binrui;ZHANG Yixuan;GAO Jian;XU Hong;LI Sha(School of Food and Light Industry,State Key Laboratory of Materials Chemistry,Nanjing Tech University,Nanjing 211800,China;College of Marine and Bioengineering,Yancheng Institute of Technology,Yancheng 224051,China)

机构地区：[1]南京工业大学材料化学国家重点实验室食品与轻工学院,江苏南京211800 [2]盐城工学院海洋与生物工程学院,江苏盐城224051

出　　处：《生物加工过程》2025年第1期42-48,共7页Chinese Journal of Bioprocess Engineering

基　　金：江苏省先进生物制造创新中心资助课题(XTD2201)。

摘　　要：多黏类芽孢杆菌(Paenibacillus polymyxa)具有抗病促生能力,在农业上得到广泛应用,芽孢特殊的抗逆性使得其方便运输和贮存,但自然条件下P.polymyxa萌发率低,限制了其推广应用。本文研究了P.polymyxa ZJ-9芽孢的热激活条件和萌发工艺,对萌发过程进行了表征。结果表明:在75℃、30 min的热激活条件下,芽孢的存活率达到98%以上且仍有较高的萌发水平;采用溶解于磷酸盐缓冲液(PBS)的L-丙氨酸+蔗糖(10.0 mmol/L)混合溶液,在室温恒温萌发12 h后,萌发率达到63.9%。对萌发过程表征发现,添加L-丙氨酸使得芽孢内2,6-吡啶二羧酸钙(DPA-Ca^(2+))释放量提高了3.35倍,利用透射电子显微镜(TEM)观察到芽孢复水现象增强,可见L-丙氨酸对芽孢萌发有明显促进作用。本研究为P.polymyxa芽孢制剂的应用提供了有益思路。Paenibacillus polymyxais is widely used in agriculture due to its excellent ability for diseaseresistance and growth promotion.The special stress resistance of spores makes them convenient for transportation and storage,however the low germination rate of P.polymyxa under natural conditions greatly limits its general application.Herein,we evaluated the thermal activation conditions and investigated the corresponding germination process of P.polymyxa ZJ-9 spores.As a result,the survival rate of spores reached over 98% under the thermal activation conditions of 75℃ for 30 min.More importantly,the subsequent treatment in a mixed solution of Lalanine and sucrose(10.0 mmol/L)in phosphate buffered saline(PBS)led to the germination rate of 63.9% after 12 hours at room temperature.Based on our investigations of the germination process,the addition of Lalanine increased the release of calcium 2,6pyridinedicarboxylate(DPACa^(2+))from spores by 3.35 times.During this process,the enhance drehydration of spores was observed by transmission electron microscopy(TEM),and L-alanine greatly promoted the spore germination.This study will be beneficial for further application of P.polymyxa spore.

关 键 词：多黏类芽孢杆菌 芽孢萌发 热激活 L-丙氨酸 

分 类 号：Q815[生物学—生物工程]