基于响应面法和神经网络优化野生狼牙刺中总黄酮的提取工艺  

Optimization of Total Flavonoids from Wild Sophora Davidii(Franch.)Skeels by Response Surface Methodology and Neural Network

作　　者：严和平[1,2] 徐世娟[1,2] 蔡朴春 张举成[1,2] Yan Heping;Xu Shijuan;Cai Puchun;Zhang Jucheng(School of Chemistry and Resources Engineering,Honghe University,Mengzi 661199,China;Honghe State Ethnic Drug Research and Development Key Laboratory of Universities in Yunnan Province,Honghe University,Mengzi 661199,China)

机构地区：[1]红河学院化学与资源工程学院,云南蒙自661199 [2]红河学院云南省高校红河州民族药物研发重点实验室,云南蒙自661199

出　　处：《黑龙江科学》2025年第2期20-25,共6页Heilongjiang Science

基　　金：云南省地方本科高校(部分)基础研究联合基金重点项目(2017FH001-003);红河学院大学生创新创业训练计划项目(CX2022131);云南省中青年学术和技术带头人培养基金(2019HB057);2024年云南省科技特派员(纵20240028);2023年云南省“三区”科技人才。

摘　　要：利用单因素试验、响应面法和神经网络-遗传寻优法对野生狼牙刺中总黄酮的提取工艺进行优化。以提取时间、料液比、乙醇浓度为变量因素,狼牙刺中总黄酮的提取率为响应指标。响应面实验获得的优化结果为乙醇浓度60%、料液比1∶30(g/mL)、提取时间90 min时,野生狼牙刺中总黄酮提取率为0.86%。以响应面数据作为学习数据,采用人工神经网络经遗传寻优获得的最优实验条件为:料液比和乙醇浓度及提取时间分别为1∶20、80%和2.22 h。在此条件下,野生狼牙刺中总黄酮的平均提取率为0.77%。从结果来看,响应面优化的实验工艺对野生狼牙刺中总黄酮的提取更有效。The study investigates the optimal extraction process of total flavonoids from Sophora davidii(Franch.)Skeels with single factor experiments,response surface methodology(RSM)analysis and genetic algorithm-neural network(GA-ANN).The extraction time,solid-liquid ratio and ethanol concentration are the variable factors,and the extraction rate of total flavonoids is the response index.The optimal results of response surface experiment are as follows:when ethanol concentration is 60%,solid-liquid ratio is 1∶30(g/mL)and extraction time is 90 min,the extraction rate of total flavonoids is 0.86%.Through using response surface data as learning data,the optimal experimental conditions obtained by genetic optimization using artificial neural network are as follows:solid-liquid ratio,ethanol concentration and extraction time are 1∶20,80%and 2.22 h,respectively.Under these conditions,the average extraction rate of total flavonoids is 0.77%.The results show that the response surface optimization technique is more effective for the extraction of total flavonoids from wild wolfsthorn.

关 键 词：响应面 神经网络 野生狼牙刺 总黄酮 

分 类 号：TQ28[化学工程—有机化工] TP18[自动化与计算机技术—控制理论与控制工程]