Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability  

作　　者：Yao Zhang Chang Xu Yun Huang Dongmei Tan Wenping Luo Yan Zhang Yi Tan 

机构地区：[1]Laboratory of Animal Center,Chongqing Medical University,Chongqing,China [2]Laboratory of Animal Center,Southwest University,Chongqing,China [3]Stomatological Hospital of Chongqing Medical University,Chongqing,China

出　　处：《Animal Models and Experimental Medicine》2024年第6期824-834,共11页动物模型与实验医学(英文)

摘　　要：Background:A stable and standardized source of mesenchymal stem cells is a prerequisite for bone repair tissue engineering research and application.We aimed to establish a stable cell line of bone marrow mesenchymal stem cells from New Zealand rabbits and explore their osteogenic differentiation capacity.Methods:Primary rabbit bone marrow mesenchymal stem cells(RBMSCs)were isolated and immortalized via retroviral expression of SV40 Large T antigen(LTA).To assess the osteogenic differentiation capacity of the cells in vitro,we studied the alkaline phosphatase(ALP)expression level and calcium deposition in bone morphogenetic protein 9(BMP9)-i nduced immortalized cells using ALP staining and quantification,as well as alizarin red staining.Ectopic bone formation by the cells was assessed using micro-computed tomography(μCT)and histological examination.Results:The immortalized cell line we established using SV40 LTA,which we termed iRBMSCs,was non-tumorigenic and maintained long-term proliferative activity.We further discovered that BMP9(MOI=30)effectively induced the osteogenic differentiation capacity of iRBMSCs in vitro,and there was a synergy with GelMA hydrogel in inducing osteogenic differentiation of the iRBMSCs in vivo.Conclusion:We confirmed that iRBMSCs are promising as a stable cell line source for bone defect repair engineering.

关 键 词：bone marrow mesenchymal stem cells bone morphogenetic protein 9 GelMA hydrogel IMMORTALIZATION 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]