High expression of stearoyl-coenzyme A desaturase in colorectal cancer oncogenic functions and its potential as a therapeutic target  

作　　者：Xiao-Wei Wang Wan-Ying Huang Kai Qin Da-Tong Zeng Zu-Yuan Chen Bang-Teng Chi Yu-Xing Tang Qi Li Bin Li Dong-Ming Li Rong-Quan He Wei-Jian Huang Gang Chen Rui-Xue Tang Zhen-Bo Feng 

机构地区：[1]Department of Pathology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China [2]Department of Pathology,Redcross Hospital of Yulin City,Yulin 537000,Guangxi Zhuang Autonomous Region,China [3]Department of Medical Oncology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China [4]Department of Pathology,The First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,Jinan 250000,Shandong Province,China

出　　处：《World Journal of Gastrointestinal Surgery》2025年第2期247-264,共18页世界胃肠外科杂志(英文)

基　　金：Supported by Natural Science Foundation of Shandong Province,No.ZR2020QH185;Scientific Research Nurturing Fund of the First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,No.QYPY2020NSFC0803;Guangxi Zhuang Autonomous Region Health Commission Scientific Research Project,No.Z20210442.

摘　　要：BACKGROUND The stearoyl-coenzyme A desaturase(SCD)gene influences colorectal cancer(CRC)pathogenesis,with its expression linked to tumor cell survival and resistance,necessitating further investigation into its role in CRC.AIM To explore the clinical and pathological significance of SCD expression in CRC tissues and to evaluate the affinity between nitidine chloride(NC)and SCD as a target.METHODS Multi-center high-throughput data related to CRC were integrated to calculate the standardized mean difference of SCD mRNA expression levels.Immunohistochemical staining results,Clustered Regularly Interspaced Short Palindromic Repeats knockout screening results of cell growth,and single-cell sequencing were employed to verify the significance of SCD expression in CRC.The clinical and pathological significance of SCD was assessed using pooled receiver operating characteristic curves,sensitivity,specificity,and likelihood ratios.The molecular mechanism of NC against CRC was clarified using the SwissTarget Prediction and functional enrichment,and molecular docking techniques were utilized to explore the targeting affinity between NC and SCD.RESULTS Data from 18 platforms,including 2482 CRC samples and 1334 non-cancerous colorectal tissue controls.SCD expression was significantly upregulated in CRC,with a standardized mean difference of 2.05[95%confidence interval(CI):1.69-2.41].The area under the pooled receiver operating characteristic curve was 0.95(95%CI:0.92-0.96),with a sensitivity of 0.86(95%CI:0.81-0.90)and a specificity of 0.90(95%CI:0.87-0.93).Positive and negative likelihood ratios were 9.02(95%CI:6.49-12.51)and 0.15(95%CI:0.10-0.22),respectively.High SCD protein expression was noted in 208 CRC patients,significantly associated with vascular invasion(P<0.001).At the singlecell level,SCD was significantly overexpressed in CRC cells(P<0.001).A total of 33 CRC cell lines depended on SCD for growth.The potential mechanism of NC against CRC might involve modulation of the cell cycle,positioning SCD as a potential targ

关 键 词：Colorectal cancer Stearoyl-coenzyme A desaturase Nitidine chloride Molecular docking Standardized mean difference 

分 类 号：R735.34[医药卫生—肿瘤]