中等强度持续运动与高强度间歇运动对高脂膳食大鼠肠道炎症及α7型烟碱乙酰胆碱受体、核转录因子κΒp65的影响  

The effects of moderate-intensity continuous training and high-intensity interval training in reducing intestinal inflammation resulting from a high-fat diet

作　　者：王伟欢 代玉玺 吴卫东[1,3] 杜凝萃 刘帅 何玉秀[1,2] Wang Weihuan;Dai Yuxi;Wu Weidong;Du Ningcui;Liu Shuai;He Yuxiu(School of Physical Education,Hebei Normal University,Shijiazhuang 050024,China;Key Laboratory of Measurement and Evaluation in Exercise Bioinformation of Hebei Province,Shijiazhuang 050024,China;Department of Phisical Education,Henan Sports University,Zhengzhou 450044,China)

机构地区：[1]河北师范大学,石家庄050024 [2]河北省人体运动生物信息测评重点实验室,石家庄050024 [3]河南体育学院,郑州450044

出　　处：《中华物理医学与康复杂志》2024年第12期1072-1078,共7页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：河北省中央引导地方科技发展资金项目(246Z5701G)。

摘　　要：目的观察12周的中等强度持续运动(MICT)与高强度间歇运动(HIIT)对高脂膳食大鼠肠道炎症及α7型烟碱乙酰胆碱受体(α7nAChR)、核转录因子κΒp65(NF-κΒp65)表达的影响。方法选取5周龄健康SD雄性大鼠32只,按照随机数字表法将其分为普通膳食安静组、高脂膳食安静组、高脂膳食MICT组、高脂膳食HIIT组,每组8只。高脂膳食MICT组以70%最大摄氧量(VO_(2)max)的强度进行持续跑台运动,高脂膳食HIIT组以40%~45%VO_(2)max和95%~99%VO_(2)max的强度交替进行跑台运动,普通膳食安静组、高脂膳食安静组不进行运动干预。12周后,采用苏木精-伊红(HE)染色观察4组大鼠的肠道组织形态;测定大鼠血清的低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、甘油三酯(TG)、游离脂肪酸(FFA)含量;采用免疫荧光双标记和Western blot法检测大鼠肠道α7nAChR、NF-κΒp65、肿瘤坏死因子α(TNF-α)的表达水平。结果与普通膳食安静组比较,高脂膳食安静组大鼠的终体重[(661.30±31.68)g]较重、总摄食量[(2011.84±77.40)g]较低,血清LDL[(65.50±20.65)mg/dL]、TG[(45.69±7.91)mg/dL]、FFA[(8.76±1.41)mg/dL]含量较高,HDL[(53.99±20.65)mg/dL]含量较低,肠道α7nAChR的蛋白表达量较低,NF-κΒp65的平均荧光强度和蛋白表达量较高,TNF-α的蛋白表达量较高(P<0.05)。与高脂膳食安静组比较,高脂膳食MICT组、高脂膳食HIIT组的终体重较轻、总摄食量较低,LDL、FFA含量均较低,α7nAChR的平均荧光强度和蛋白表达量增加,NF-κΒp65的平均荧光强度和的蛋白表达量降低,TNF-α的蛋白表达量较低(P<0.05)。与高脂膳食MICT组比较,高脂膳食HIIT组的HDL含量[(67.27±23.32)mg/dL]较高,NF-κΒp65的平均荧光强度较低(P<0.05)。高脂膳食MICT组和高脂膳食HIIT组大鼠的结肠组织切片,表现为炎性浸润减轻,上皮损伤及黏膜隐窝破坏减少。结论12周的MICT和HIIT均可改善高脂膳食大鼠的肠道炎症状态,增加肠道α7nAChR蛋白�ObjectiveTo observe any effect of 12 weeks of moderate-intensity continuous training(MICT)and of high-intensity interval training(HIIT)on intestinal inflammation and the expression ofα7 nicotinic acetylcholine receptor(α7nAChR)and nuclear factor kappa B p65(NF-κΒp65)in rats fed a high-fat diet.MethodsThirty-two healthy 5-week-old male Sprague-Dawley rats were divided at random into a normal diet and quiet group,a high-fat diet and quiet group,a high-fat diet MICT group,and a high-fat diet HIIT group,each of 8.The high-fat diet MICT group underwent continuous treadmill exercise at 70%of their maximum oxygen uptake(VO_(2)max),while the high-fat diet HIIT group did treadmill exercise at 40%to 45%of their VO_(2)max alternating with intervals at 95%to 99%of their VO_(2)max.The two quiet groups did no exercise.After 12 weeks,hematoxylin-eosin staining was used to observe the morphology of the rats′intestinal tissue.Serum levels of low-density lipoprotein(LDL),high-density lipoprotein(HDL),triglycerides(TG)and free fatty acids(FFA)were determined,and immunofluorescence double labeling and western blotting were applied to detect the expression levels ofα7nAChR,NF-κB p65 and tumor necrosis factorα(TNF-α)in the rats′intestines.ResultsCompared with the normal diet quiet group,the quiet group on a high fat diet had,on average,significantly heavier final body weights,lower total food intake,higher serum LDL,TG and FFA,lower HDL levels,and less protein expression of intestinalα7nAChR.They showed higher average fluorescence intensity and expression of NF-κB p65 and TNF-αprotein.Compared with the high-fat diet quiet group,the high-fat diet MICT and HIIT groups had significantly lighter final body weight,on average,lower total food intake,lower LDL and FFA levels and higher average fluorescence intensity.They showed significantly greater expression ofα7nAChR and NF-κB p65 protein,and lower expression of TNF-αprotein.Compared with the high-fat diet MICT group,there was a significantly higher HDL content and lower

关 键 词：肠道炎症 核转录因子κΒp65 肿瘤坏死因子Α α7型烟碱乙酰胆碱受体 高强度间歇运动 高脂膳食 

分 类 号：R574[医药卫生—消化系统] G804.2[医药卫生—内科学]