AGEs-RAGE介导颞下颌关节软骨细胞凋亡的初步探讨  

Preliminary study on chondrocyte apoptosis mediated by AGEs-RAGE in temporomandibular joint

作　　者：张懿丹 蔡璐璇 扈宁 王雪东[1] ZHANG Yi-dan;CAI Lu-xuan;HU Ning;WANG Xue-dong(Department of Orthodontics,Peking University School and Hospital of Stomatology,Beijing 100081,China)

机构地区：[1]北京大学口腔医学院正畸科、国家口腔医学中心、国家口腔疾病临床医学研究中心、口腔数字化医疗技术和材料国家工程实验室、口腔数字医学北京市重点实验室,北京100081

出　　处：《北京口腔医学》2024年第6期387-392,共6页Beijing Journal of Stomatology

基　　金：国家自然科学基金(81671015,8237030822)。

摘　　要：目的探讨晚期糖基化终末产物及其受体(AGEs-RAGE)调控颞下颌关节骨关节炎(TMJOA)病变早期阶段软骨细胞凋亡的分子机制。方法体内实验采用碘乙酸钠颞下颌关节注射和咬合干扰的方法建立大鼠TMJOA动物模型;通过HE染色评价软骨病变,通过TUNEL染色检测软骨细胞凋亡,通过免疫组化检测AGEs、RAGE水平变化。体外实验在软骨细胞中加入AGEs通过TUNEL染色和蛋白免疫印迹检测细胞凋亡变化。结果在两种TMJOA大鼠模型中,早期TUNEL染色阳性的凋亡软骨细胞显著增加,伴随AGEs、RAGE表达上调。AGEs可以独立诱导软骨细胞凋亡,伴随caspase-3的活化增加。结论AGEs-RAGE参与TMJOA病变早期的软骨细胞凋亡。Objective To investigate the molecular mechanism of advanced glycation end products and their receptors(AGEs-RAGE)regulating chondrocyte apoptosis in the early stage of temporomandibular osteoarthritis(TMJOA).Methods In vivo,TMJOA model was established by Monosodium Iodoacetate(MIA)injection into TMJ and occlusal interference.Cartilage lesions were evaluated by HE staining,chondrocyte apoptosis was detected by TUNEL staining,and AGEs and RAGE levels were detected by immunohistochemistry.In vitro,AGEs were added to chondrocytes and apoptosis was detected by TUNEL staining and western blotting.Results The apoptotic chondrocytes in the two early TMJOA rat models were significantly increased,along with up-regulated expression of AGEs and RAGE.AGEs can independently induce chondrocyte apoptosis,accompanied by increased activation of caspase-3.Conclusions AGE-RAGE is involved in chondrocyte apoptosis in the early stage of TMJOA.

关 键 词：AGEs-RAGE 颞下颌关节骨关节炎 软骨细胞凋亡 

分 类 号：R782.6[医药卫生—口腔医学]