急性缺血性脑卒中患者血清LncRNA RMST,miR-582-5p表达水平与早期神经功能恶化及预后的相关性研究  

Correlation between Serum LncRNA RMST,miR-582-5p Expression Levels and Early Neurological Deterioration and Prognosis in Patients with Acute Ischemic Stroke

作　　者：任建华[1] 张然[2] 崔秀英 赵新 REN Jianhua;ZHANG Ran;CUI Xiuying;ZHAO Xin(Department of Neurodiagnosis,Chengde Central Hospital,Hebei Chengde 067000,China;Department of Neurology,Chengde Central Hospital,Hebei Chengde 067000,China)

机构地区：[1]承德市中心医院神经诊断科,河北承德067000 [2]承德市中心医院神经内科,河北承德067000

出　　处：《现代检验医学杂志》2025年第1期64-68,共5页Journal of Modern Laboratory Medicine

基　　金：承德市科学技术研究与发展计划项目(编号:202204A010)。

摘　　要：目的 探讨急性缺血性脑卒中(AIS)患者血清长链非编码RNA横纹肌肉瘤相关转录物-2(LncRNA RMST)和miR-582-5p的表达水平与早期神经功能恶化(END)及预后的相关性。方法 选取2021年1月~2022年12月期间承德市中心医院收治的129例AIS患者,并根据患者一周内是否出现END将患者分为END组(n=42)和非END组(n=87),另选取同期在该院体检的健康者59例作为健康组。采用qRT-PCR法检测各组血清LncRNA RMST,miR-582-5p水平。Pearson法对END患者血清LncRNA RMST,miR-582-5p水平相关性进行分析;绘制ROC曲线评估血清LncRNA RMST,miR-582-5p水平以及二者联合对AIS患者预后情况的效能分析。结果 健康组、非END组、END组血清LncRNA RMST水平(1.01±0.28,2.10±0.41,3.99±0.52)逐渐升高,miR-582-5p水平(1.02±0.23,0.86±0.16,0.73±0.15)逐渐降低,差异具有统计学意义(F=672.974,31.907,均P <0.05)。END患者血清LncRNA RMST水平与mi R-582-5p水平存在明显的负相关(r=-0.451,P<0.001)。血清LncRNA RMST,miR-582-5p水平联合诊断AIS患者预后的AUC(95%CI)为0.961(0.912~0.987),优于单独预测(Z=2.280,4.515,均P <0.05)。结论 血清LncRNA RMST,miR-582-5p水平与END关系密切,有望成为AIS患者预后的预测因子。Objective To investigate the expression changes of serum long non-coding RNA rhabdomyosarcoma associated transcript-2(LncRNA RMST)and miR-582-5p in patients with acute ischemic stroke(AIS),and their correlation with early neurological deterioration(END)and prognosis.Methods A total of 129 patients with ischemic stroke admitted to Chengde Central Hospital from January 2021 to December 2022 were gathered,according to diagnostic criteria and patients were divided into END group(n=42)and non END group(n=87)besed on whether END occurred within one week.In addition,59 healthy individuals who underwent physical examination in the hospital were regarded as the health group.The real-time fluorescence quantitative polymerase chain reaction method was applied to detect serum LncRNA RMST and miR-582-5p levels in each group.The Pearson method was used to analyze the correlation between serum LncRNA RMST and miR-582-5p levels in END patients.ROC curve was applied to evaluate further the levels of blood LncRNA RMST and miR-582-5p,and the efficacy analysis of their combination in predicting the prognosis of AIS patients.Results Serum LncRNA RMST(1.01±0.28,2.10±0.41,3.99±0.52)levels gradually increased in the healthy,non-END and END groups,and miR-582-5p(1.02±0.23,0.86±0.16,0.73±0.15)levels gradually decreased,and the differences were statistically significant(F=672.974,31.907,all P<0.05).There was a significant negative correlation between serum LncRNA RMST levels were significantly negatively correlated with miR-582-5p levels(r=-0.451,P<0.001).The AUC(95%CI)of the combined diagnosis of serum LncRNA RMST and miR-582-5p levels in AIS patients was 0.961(0.912~0.987),which was better than the independent prediction(Z=2.280,4.515,all P<0.05).Conclusion The levels of serum LncRNA RMST and miR-582-5p were closely related to END,and they are expected to become predictive factors for the prognosis of AIS patients.

关 键 词：长链非编码RNA横纹肌肉瘤相关转录物-2 微小RNA-582-5p 急性缺血性脑卒中 神经功能恶化 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R392.11[医药卫生—临床医学]