三氯化铁损伤小鼠颈动脉血栓模型的影响因素  

Influencing Factors of FeCl_3 Induced Mouse Carotid Artery Thrombosis Model

作　　者：杜佳豪 赵丽丽[1] 杨标 戴克胜[1] DU Jia-Hao;ZHAO Li-Li;YANG Biao;DAI Ke-Sheng(Suzhou Medical College of Soochow University,Jiangsu Institute of Hematology,The First Affiliated Hospital of Soochow University,Key Laboratory of Thrombosis and Hemostasis of Ministry of Health,State Key Laboratory of Radiation Medicine and Protection,Collaborative Innovation Center of Hematology,Suzhou 215006,Jiangsu Province,China)

机构地区：[1]苏州大学苏州医学院,江苏省血液研究所,苏州大学附属第一医院,卫生部血栓与止血重点实验室,放射医学与防护国家重点实验室,血液学协同创新中心,江苏苏州215006

出　　处：《中国实验血液学杂志》2025年第1期193-197,共5页Journal of Experimental Hematology

基　　金：基础研究计划自然科学基金(BK20221236)。

摘　　要：目的:探究构建三氯化铁(FeCl_(3))损伤小鼠颈动脉血栓模型的主要影响因素。方法:FeCl_(3)损伤颈总动脉后,将损伤后血管取下进行固定、包埋、冷冻切片和HE染色,并统计血栓面积比。FeCl_(3)浓度分5%、10%和15%3组,FeCl_(3)损伤时间选取2、4和6 min 3组,小鼠的周龄分4-5、6-8和10周3组。0.075μg/g和0.1μg/g剂量R300注入小鼠眼球后静脉丛,4 h后进行血小板计数,探究血小板数量对FeCl_(3)诱导血栓模型的影响,验证构建的血栓模型的准确性。结果:HE染色观察显示,与5%FeCl_(3)诱导的血栓相比,10%和15%FeCl_(3)氧化损伤血管诱导形成的血栓块更稳定,致密且更大。10%FeCl_(3)损伤血管内皮4 min和6 min后诱导形成致密和大的血栓块,而损伤2 min时诱导形成的血栓更松散且血栓面积小。选取的4-5、6-8和10周龄的小鼠在对10%FeCl_(3)损伤诱导形成的致密血栓和血栓面积差异上并不明显。结论:FeCl_(3)损伤的小鼠血栓模型受到许多因素的影响。10%FeCl_(3)、损伤时间4 min、6-8周龄小鼠作为最佳的实验条件,对于构建稳定的颈动脉血栓模型是合适的。Objective:To investigate the factors affecting ferric chloride(FeCl_(3))-induced carotid artery thrombosis model experiment.Methods:After the common carotid artery was damaged by FeCl_(3),the injured vessels were dissected for fixation,embedding,frozen section,and then processed HE staining.The carotid thrombus area ratio was calculated.We examined the effect of FeCl_(3) concentration(5%,10% and 15%),reaction time(2,4 and 6 min),and recipient mouse age(4-5,6-8 and 10 weeks) on the formation and stability of arterial thrombosis model.The model was injected through the post-glomus venous plexus of mouse eyeball with 0.075 μg/g and 0.1μg/g R300 to verify the accuracy of the FeCl_(3)-induced model on thrombus formation by adjusting the platelet number.Results:HE staining showed that thrombus formation induced by 10% and 15% FeCl_(3) was more stable,dense and larger than 5% FeCl_(3)-induced thrombosis.10%FeCl_(3) induced the formation of dense and large thrombosis after 4 and 6 minutes of vascular endothelium injury,while the thrombosis induced for 2 minutes were looser and smaller in area.Mouse age can not affect thrombus formation and stability,because there were no significant differences in the formation of dense thrombus and thrombus area induced by 10% FeCl_(3) among three different age groups of mice.Conclusion:Many factors affect the formation and stability of arterial thrombosis model induced by FeCl_(3).This optimal experimental conditions for construction of a stable carotid artery thrombosis model are 10% FeCl_(3),4 minutes for injury,and 6-8 week old mice.

关 键 词：血小板 颈动脉 三氯化铁 

分 类 号：R364.15[医药卫生—病理学]