长链非编码RNA LINC00926调控微小RNA-331-3p对前列腺癌细胞增殖和迁移的影响  

Effect of lncRNA LINC00926 on the proliferation and migration of prostate cancer cells by regulating miR-331-3p

作　　者：程树林[1] 杜中波 黄静[1] 张强 李建勇[1] CHENG Shulin;DU Zhongbo;HUANG Jing;ZHANG Qiang;LI Jianyong(Department of Urology,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院附属医院泌尿外科,四川南充637000

出　　处：《陕西医学杂志》2025年第2期170-174,180,共6页Shaanxi Medical Journal

基　　金：四川省卫生健康科研课题(20PJ154)。

摘　　要：目的:探讨长链非编码RNA(lncRNA)LINC00926对前列腺癌细胞增殖和迁移的影响及分子机制。方法:前列腺癌组织和癌旁组织LINC00926表达用LncRNADisease数据库分析。采用RT-qPCR检测正常前列腺上皮RWPE-1细胞和前列腺癌细胞株22Rv1、PC3、C4-2B、LNCaP、DU-145中LINC00926的表达。选取LINC00926表达最高的前列腺癌DU-145细胞,分别转染LINC00926小干扰RNA(si-LINC00926组)和阴性对照RNA(si-NC组)。采用CCK-8法、划痕实验检测DU-145细胞增殖和迁移能力。双荧光素酶报告基因实验检测LINC00926与miR-331-3p的靶向结合。采用RT-qPCR检测DU-145细胞中miR-331-3p表达。采用Western blot检测Wnt/β-连环蛋白(β-catenin)信号通路相关蛋白表达。结果:前列腺癌组织LINC00926表达水平高于癌旁组织(P<0.05)。与RWPE-1细胞比较,LINC00926在前列腺癌22Rv1、PC3、DU-145、C4-2B、LNCaP细胞中表达水平升高,且DU-145细胞表达最高(均P<0.05)。si-NC组LINC00926表达水平高于si-LINC00926组(P<0.05)。于24、48、72、96 h,si-LINC00926组DU-145细胞增殖能力低于si-NC组(均P<0.05)。与si-NC组比较,si-LINC00926组DU-145细胞迁移能力下降(P<0.05)。LINC00926-WT和miR-331-3p共转染荧光素酶相对活性低于LINC00926-WT和miR-NC共转染(P<0.05)。si-NC组miR-331-3p表达水平低于si-LINC00926组(P<0.05)。与si-NC组比较,si-LINC00926组DU-145细胞中β-连环蛋白(β-catenin)、细胞髓细胞瘤病毒癌基因同源物蛋白(c-myc)、细胞周期蛋白D1(Cyclin D1)、周期蛋白依赖激酶3(CDK3)、B淋巴细胞瘤-2(Bcl-2)蛋白表达水平降低(均P<0.05)。结论:LINC00926在前列腺癌组织和细胞中高表达,下调LINC00926可能通过靶向miR-331-3p抑制前列腺癌细胞的增殖和迁移能力。Objective:To explore the effect and mechanisms of lncRNA LINC00926 on the proliferation and migration of prostate cancer cells.Methods:The expression of LINC00926 in prostate cancer tissue and adjacent non-cancerous tissue was analyzed using the LncRNADisease database.The expression of LINC00926 in RWPE-1 cells and prostate cancer cell lines 22Rv1,PC3,C4-2B,LNCaP,and DU-145 was detected by RT-qPCR.DU-145 cells,which had the highest expression of LINC00926 among the prostate cancer cells,were transfected with LINC00926 siRNA(si-LINC00926 group)and negative control RNA(si-NC group).The proliferation and migration abilities of DU-145 cells were detected by CCK-8 assay and scratch test.The targeting binding of LINC00926 to miR-331-3p was detected by dual-luciferase reporter gene assay.The expression of miR-331-3p in DU-145 cells was detected by RT-qPCR.The expression of proteins related to the Wnt/β-catenin signaling pathway was detected by Western blot.Results:The expression level of LINC00926 in prostate cancer tissue was higher than that in adjacent non-cancerous tissue(P<0.05).Compared with RWPE-1 cells,the expression of LINC00926 levels in prostate cancer cell lines 22Rv1,PC3,DU-145,C4-2B and LNCaP were increased,with the highest expression in DU-145 cells(all P<0.05).The expression level of LINC00926 in the si-NC group was higher than that in the si-LINC00926 group(P<0.05).At 24,48,72 and 96 hours,the proliferation ability of DU-145 cells in the si-LINC00926 group was lower than that in the si-NC group(all P<0.05).Compared with the si-NC group,the migration ability of DU-145 cells in the si-LINC00926 group was decreased(P<0.05).The relative activity of the luciferase co-transfected with LINC00926-WT and miR-331-3p was lower than that co-transfected with LINC00926-WT and miR-NC(P<0.05).The expression level of miR-331-3p in the si-NC group was lower than that in the si-LINC00926 group(P<0.05).Compared with the si-NC group,the protein expression levels ofβ-catenin,c-myc,cyclin D1,CDK3 and Bcl-2 in DU-145 cells i

关 键 词：前列腺癌 长链非编码RNA LINC00926 微小RNA-331-3p 增殖 迁移 

分 类 号：R36[医药卫生—病理学]