Transcriptome Analysis of Derris fordii and Derris elliptica to Identify Potential Genes Involved in Rotenoid Biosynthesis  

作　　者：Yanlin Pan Yibin Zhang Xingui Wang Hongbo Qin Lunfa Guo 

机构地区：[1]Guangxi Institute of Botany,Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences,Guilin,541006,China [2]Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain,Guangxi Institute of Botany,Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences,Guilin,541006,China

出　　处：《Phyton-International Journal of Experimental Botany》2025年第1期123-136,共14页国际实验植物学杂志(英文)

基　　金：Guangxi Science and Technology Base and Talent Special Fund,Project No.AD21220130;Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain,Project No.20-065-7;Guangxi Institute of Botany Fund,Project No.21014.

摘　　要：Derris fordii and Derris elliptica belong to the Derris genus of the Fabaceae family, distinguished by their high isoflavonoid content, particularly rotenoids, which hold significance in pharmaceuticals and agriculture. Rotenone, as a prominent rotenoid, has a longstanding history of use in pesticides, veterinary applications, medicine, and medical research. The accumulation of rotenoids within Derris plants adheres to species-specific and tissue-specific patterns and is also influenced by environmental factors. Current research predominantly addresses extraction techniques, pharmacological applications, and pesticide formulations, whereas investigations into the biosynthesis pathway and regulatory mechanism of rotenoids remain relatively scarce. In this study, we observed notable differences in rotenone content across the roots, stems, and leaves of D. fordii, as well as within the roots of D. elliptica. Utilizing RNA sequencing (RNA-seq), we analyzed the transcriptomes and expression profiles of unigenes from these four tissues, identifying a total of 121,576 unigenes. Differentially expressed genes (DEGs) across four comparison groups demonstrated significant enrichment in the phenylpropanoid and flavonoid biosynthesis pathways. Key unigenes implicated in the rotenoid biosynthesis pathway were identified, with PAL, C4H, CHS, CHI, IFS, and HI4OMT playing critical roles in D. fordii, while IFS and HI4OMT were determined to be essential for rotenoid biosynthesis in D. elliptica. These findings enhance our understanding of the biosynthesis mechanism of rotenoids in Derris species. The unigenes identified in this study represent promising candidates for future investigations aimed at validating their roles in rotenoid biosynthesis.

关 键 词：Derris fordii Derris elliptica RNA-SEQ rotenoid comparative transcriptomic analysis 

分 类 号：Q94[生物学—植物学]