Exogenous Alpha-Ketoglutarate(AKG)Modulate Physiological Characteristics,Photosynthesis,Secondary Metabolism and Antioxidant Defense System in Peganum Harmala L.under Nickel Stress  

作　　者：Marwa Rezgui Wided Ben Ammar Muhammad Nazim Walid Soufan Chiraz Chaffei Haouari 

机构地区：[1]Department of Biology,University of Tunis El Manar,Laboratory of Vegetable Productivity and Environmental Constraints,Faculty of Sciences of Tunis(LR18ES04),University of Tunis El Manar(UTM),Tunis,1060,Tunisia [2]State Key Laboratory of Desert and Oasis Ecology,Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi,830011,China [3]University of Chinese Academy of Sciences,Beijing,100049,China [4]Plant Production Department,College of Food and Agriculture Sciences,King Saud University,Riyadh,11451,Saudi Arabia

出　　处：《Phyton-International Journal of Experimental Botany》2025年第1期137-155,共19页国际实验植物学杂志(英文)

基　　金：Researchers Supporting Project No.(RSP2025R390),King Saud University,Riyadh,Saudi Arabia.

摘　　要：Nickel(Ni)toxicity significantly impairs plant growth,photosynthesis,and metabolism by inducing oxidative stress.This study evaluates the potential of exogenous Alpha-Ketoglutarate(AKG)in mitigating Ni-induced stress in Peganum harmala L.Seedlings were exposed to 0,200,500,and 750μM NiCl2,with or without AKG supplementation.Under 750μM Ni stress,dry weight(DW)decreased by 33.7%,tissue water content(TWC)by 39.9%,and chlorophyll a and total chlorophyll levels were reduced by 17%and 15%,respectively.Ni exposure also significantly increased secondary metabolite production,with leaf anthocyanin content rising by 131%,and superoxide dismutase(SOD)and catalase(CAT)activities increasing by 228%and 53%,respectively,in roots at 500μM Ni.AKG treatment alleviated Ni toxicity by enhancing TWC by 39%and promoting root and shoot growth.Additionally,AKG treatment boosted the synthesis of phenolic compounds and flavonoids,contributing to improved tolerance against Ni stress.These findings demonstrate the potential of AKG in enhancing Ni tolerance in P.harmala,suggesting its promising role in bioremediation of metal-contaminated soils.This is the first study to report the beneficial effects of exogenous AKG in alleviating nickel toxicity in P.harmala L.,offering a new approach for improving plant resilience to heavy metal stress.

关 键 词：ALPHA-KETOGLUTARATE antioxidative enzymes oxidative stress Peganum harmala NICKEL 

分 类 号：Q94[生物学—植物学]