MiR-186抑制人宫颈癌HeLa细胞增殖和PI3K/AKT信号通路活性  

MiR-186 inhibits proliferation and activity of PI3K/AKT signaling pathway in human cervical cancer HeLa cells

作　　者：李太升 刘佳 王英 Li Taisheng;Liu Jia;Wang Ying(Department of Pathology,Mudanjiang Maternal and Child Health Hospital,Mudanjiang 157000,China;Department of Infectious Diseases,Mudanjiang Maternal and Child Health Hospital,Mudanjiang 157000,China)

机构地区：[1]牡丹江市妇幼保健院病理科,牡丹江157000 [2]牡丹江市妇幼保健院感染科,牡丹江157000

出　　处：《中国组织化学与细胞化学杂志》2024年第4期347-353,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：2018年黑龙江省卫生计生委科研课题(2018317)。

摘　　要：目的探究miR-186通过PI3K/AKT信号通路对人宫颈癌细胞增殖、克隆形成的影响。方法应用miR-186 mimic或miR-186 inhibitor改变人宫颈癌HeLa细胞系miR-186表达水平,采用实时荧光定量PCR、5-乙炔基-2’脱氧尿嘧啶核苷(EdU)、细胞计数试剂盒-8(CCK-8)、平板克隆形成法及蛋白免疫印迹法对miR-186表达量、增殖率、活力、克隆形成率和磷脂酰肌醇-3激酶/丝氨酸苏氨酸蛋白激酶(PI3K/AKT)通路相关蛋白及细胞周期蛋白(cyclin D1)、增殖细胞核抗原(PCNA)表达水平进行检测。结果miR-186 mimic显著抑制HeLa细胞增殖率、活力和克隆形成率,显著降低HeLa细胞cyclin D1、PCNA及PI3K/AKT通路蛋白p-PI3K和p-AKT蛋白水平,而miR-186 inhibitor则与miR-186 mimic作用相反。结论miR-186可能通过抑制PI3K/AKT信号通路抑制宫颈癌细胞的增殖。Objective To investigate the effect of miR-186 on the proliferation and colony formation of human cervical cancer cells through the PI3K/AKT signaling pathway.Methods MiR-186 expression in HeLa human cervical cancer cells was altered using miR-186 mimic or miR-186 inhibitor.Real-time quantitative PCR,5-ethynyl-2’-deoxyuridine(EdU)assay,Cell Counting Kit-8(CCK-8),colony formation assay,and Western blotting were used to assess miR-186 expression levels,proliferation rate,cell viability,colony formation rate,as well as the expression levels of PI3K/AKT pathway-related proteins,including cyclin D1 and proliferating cell nuclear antigen(PCNA).Results MiR-186 mimic significantly inhibited the proliferation rate,viability,and colony formation rate of HeLa cells,and significantly reduced the protein levels of cyclin D1,PCNA,and PI3K/AKT pathway proteins,including p-PI3K and p-AKT.Conversely,the miR-186 inhibitor showed the opposite effect to that of the miR-186 mimic.Conclusion MiR-186 may inhibit the proliferation of cervical cancer cells by suppressing the PI3K/AKT signaling pathway.

关 键 词：宫颈癌 miR-186 磷脂酰肌醇3激酶 蛋白激酶B 细胞增殖 

分 类 号：R73-3[医药卫生—肿瘤]