植物乳杆菌W1荚膜多糖结构、免疫及抗衰老活性的研究  

Structure,Immune and Anti-aging Activities of Capsular Polysaccharide Produced by Lactobacillus plantarum W1

作　　者：高永娇 王坤 赵婧[1,2] 左锋[1,3] GAO Yongjiao;WANG Kun;ZHAO Jing;ZUO Feng(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163000,China;National Coarse Cereals Engineering Research Center,Heilongjiang Bayi Agricultural University,Daqing 163000,China;Engineering Research Center for Processing and Utilization of Grain By-products,Ministry of Education,Daqing 163000,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163000 [2]黑龙江八一农垦大学国家杂粮工程技术中心,黑龙江大庆163000 [3]粮食副产物加工与利用教育部工程研究中心,黑龙江大庆163000

出　　处：《食品工业科技》2025年第4期90-99,共10页Science and Technology of Food Industry

基　　金：黑龙江省自然科学基金优秀青年项目(YQ2021C029);黑龙江省普通高校青年创新人才项目(UNPYSCT-2018082)。

摘　　要：为了初步探究植物乳杆菌W1荚膜多糖(CPS)的结构及生物活性。本研究采用酶解法提取制备CPS,通过凝胶渗透色谱系统、傅里叶红外光谱和高效液相色谱法分析其绝对分子量、官能团和单糖组成,并利用RAW264.7细胞和秀丽隐杆线虫模型,评估CPS的免疫和抗衰老活性。结果表明,CPS是一种平均分子量为5.49×10^(4) Da,由葡萄糖和半乳糖组成的杂多糖,其单糖摩尔比为3.12:1。CPS可以促进RAW264.7细胞的增殖和对中性红的吞噬作用,促进NO和细胞因子的分泌,从而发挥免疫调节作用;125μg/mL剂量下,RAW264.7细胞的TNF-α、IL-6、IL-1β的分泌水平达到最大值,分别为对照组的1.26倍、1.11倍、1.96倍。同时,CPS可显著提升线虫的抗氧化体系(P<0.05),延长其寿命,并提高其应激耐受性。正常、氧化应激和热休克环境下线虫的平均寿命均在125μg/mL处理剂量下达到最大值,寿命延长率分别为21.05%±0.21%、42.86%±0.27%和50.00%±0.27%。以上研究结果表明CPS具有良好的生物活性,具有开发为功能性辅料应用于食品中的潜力。In order to preliminarily explore the structure,immune and anti-aging activity of capsular polysaccharide(CPS)produced by Lactobacillus plantarum W1.In this study,CPS was extracted and prepared by enzymatic hydrolysis.The absolute molecular weight,functional groups and monosaccharide composition of CPS were analyzed by gel permeation chromatography system,Fourier transform infrared spectroscopy and high performance liquid chromatography.The immune and anti-aging activities of CPS were investigated using RAW264.7 cells and Caenorhabditis elegans(C.elegans)models.The results showed that CPS was a heteropolysaccharide with an average molecular weight of 5.49×10^(4)Da,consisting of glucose and galactose in a molar ratio of 3.12:1.Furthermore,CPS had been found to enhance the proliferation and phagocytosis of RAW264.7 cells,as well as promote the secretion of NO and cytokines,thereby exerting an immunomodulatory effect.At a concentration of 125μg/mL,the secretion levels of TNF-α,IL-6 and IL-1βin RAW264.7 cells reached their maximum values,which were 1.26 times,1.11 times and 1.96 times higher than those in the control group,respectively.Concurrently,CPS significantly enhanced the antioxidant system of C.elegans(P<0.05),prolonged their lifespan,and improved their stress tolerance.The average lifespan of nematodes under normal,oxidative stress and heat shock conditions reached its maximum at 125μg/mL,with lifespan extension rates of 21.05%±0.21%,42.86%±0.27%and 50.00%±0.27%,respectively.These findings indicate that CPS exhibits significant biological activity and has the potential to be utilized as functional excipients in food products.

关 键 词：荚膜多糖 巨噬细胞 免疫活性 秀丽隐杆线虫 

分 类 号：Q71[生物学—分子生物学]