玫瑰蜂花粉破壁工艺优化及其醇提物生理活性比较  

Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts

作　　者：卢新阳 孙奕舒 徐涛 范龙彬 常雅宁[1] 周英骏 LU Xinyang;SUN Yishu;XU Tao;FAN Longbin;CHANG Yaning;ZHOU Yingjun(College of Biotechnology,East China University of Science and Technology,Shanghai 200237,China;Huzhou Shengtao Biotechnology Co.,Ltd.,Huzhou 313000,China;Shanghai Hongcheng Industrial Development Co.,Ltd.,Shanghai 200232,China)

机构地区：[1]华东理工大学生物工程学院,上海200237 [2]湖州圣涛生物技术有限公司,浙江湖州313000 [3]上海宏成实业发展有限公司,上海200232

出　　处：《食品工业科技》2025年第4期176-184,共9页Science and Technology of Food Industry

摘　　要：玫瑰蜂花粉气味芳香、口感清甜,富含多酚等活性物质,是极具营养价值和开发潜力的天然保健品。本研究以玫瑰蜂花粉为研究对象,旨在优化其酶解破壁工艺条件,并比较不同乙醇提取物的生理活性及主要成分含量,以期筛选抗炎和抗良性前列腺增生(Benign prostatic hyperplasia,BPH)活性最佳的玫瑰蜂花粉提取物。结果显示,玫瑰蜂花粉双酶解法破壁最优工艺参数是料液比为1:30、温度55℃、pH6、复合酶体系添加量2.0%(纤维素酶:复合蛋白酶=7:5),反应9 h,可得最佳破壁率为73.13%;90%乙醇提取的活性物质能显著(P<0.01)降低脂多糖诱导的RAW264.7细胞炎症因子释放水平(20μg/mL时,与造模组相比,一氧化氮、白细胞介素-6和肿瘤坏死因子-α分别降低了67.04%、16.74%、21.74%)并对BPH-1细胞活力和增殖有最明显的抑制作用,因此具有最佳的抗炎和抗BPH效果。本研究为明确玫瑰蜂花粉中的活性成分及进一步开发其深加工产品提供了理论依据。Rose bee pollen has a pleasant aroma and a sweet taste,and it is rich in polyphenols and other bioactive substances,making it a highly nutritious and promising natural health product.To optimize the enzymatic hydrolysis process for rose bee pollen and to systematically compare the physiological activities and primary component profiles of its different ethanolic extracts.This was done with the aim of discerning the most efficacious extract that possessed significant anti-inflammatory and anti-benign prostatic hyperplasia(BPH)effects.The results demonstrated that the optimal enzymatic hydrolysis process parameters for rose bee pollen were a solid-to-liquid ratio of 1:30,a temperature of 55°C,pH6,a composite enzyme system addition of 2.0%(cellulase comprising a ratio of 7:5 with composite protease),and a reaction time of 9 h,resulting in the highest wall-disruption rate of 73.13%.The active substances extracted with 90%ethanol could significantly(P<0.01)reduce the release level of inflammatory factors induced by lipopolysaccharide in RAW264.7 cells(at 20μg/mL,compared with the model group,nitric oxide,interleukin-6 and tumor necrosis factor-αdecreased by 67.04%,16.74%and 21.74%respectively),and had the most obvious inhibitory effect on the activity and proliferation of BPH-1 cells,thus exhibiting optimal anti-inflammatory and anti-BPH effects.This research provides a theoretical basis for identifying the active ingredients in rose bee pollen and further developing value-added processed products.

关 键 词：玫瑰蜂花粉 破壁 乙醇提取物 抗炎 良性前列腺增生 

分 类 号：S896.4[农业科学—特种经济动物饲养]