防风多糖部分酸水解降解产物的免疫调节活性与结构研究  

Immunoregulatory Activity and Structure Identification of Partial Acid Hydrolysis Degradation Products of Saposhnikoviae Radix Polysaccharides

作　　者：徐畅 李洁[1] 孙萌[1] 范海涛[2] 张薇 姜艳艳[1,3] 刘斌 XU Chang;LI Jie;SUN Meng;FAN Haitao;ZHANG Wei;JIANG Yanyan;LIU Bin(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Biological Engineering College,Beijing Polytechnic,Beijing 100176,China;Key Laboratory of"Discovery of Effective Substances in Classical Prescriptions of Traditional Chinese Medicine",National Administration of Traditional Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京电子科技职业学院生物工程学院,北京100176 [3]国家中医药管理局中药经典名方有效物质发现重点研究室,北京102488

出　　处：《中国现代中药》2025年第1期71-79,共9页Modern Chinese Medicine

基　　金：国家自然科学基金项目(82074283);国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023258)。

摘　　要：目的:基于防风多糖(SP)的部分酸水解降解产物探究其免疫调节活性与结构特征。方法:选取不同浓度的三氟乙酸对SP进行控制性降解;采用长春瑞滨(NVB)诱导的免疫低下斑马鱼模型评价SP酸水解降解产物的免疫调节活性;基于亲水作用色谱-蒸发光散射法(HILIC-ELSD)和超高效液相色谱-四极杆-静电场轨道离子阱串联质谱法(UHPLC-Q-Orbitrap MS/MS)初步分析SP降解产物。结果:三氟乙酸能够有效裂解SP的糖苷键,得到丰富的寡糖片段;不同条件下降解的SP酸水解降解产物对NVB造成的免疫细胞损伤均具有治疗作用。酸水解条件越剧烈,降解生成的寡糖片段越丰富,提高斑马鱼免疫细胞密度的能力越显著。在寡糖片段最丰富的样品中共鉴定出42个酸水解降解产物,包括8个单糖、13个糖醛酸、21个寡糖(聚合度为2~5)。结论:通过酸水解方法降解结构复杂的多糖,测定相应的免疫调节活性,可为多糖的构效关系研究提供依据。Objective:To explore the immunoregulatory activity and structural characteristics of Saposhnikoviae Radix polysaccharides(SP)based on their partial acid hydrolysis degradation products.Methods:Controlled degradation of SP was carried out using trifluoroacetic acid(TFA)at different concentrations.The immunoregulatory activity of acid hydrolysis degradation products of SP were evaluated using a zebrafish immunosuppression model induced by vinorelbine(NVB).Preliminary analysis of SP degradation products was conducted using hydrophilic interaction chromatography-evaporative light scattering detector(HILIC-ELSD)and ultra-high-performance liquid chromatography-Q Exactive quadrupole-electrostatic field orbitrap tandem mass spectrometry(UHPLC-Q-Orbitrap MS/MS).Results:TFA effectively cleaved the glycosidic bonds of SP,yielding abundant oligosaccharide fragments.The SP acid hydrolysis degradation products under different conditions exhibited therapeutic effects on NVB-induced immune cell injury.If the acid hydrolysis conditions were more severe,the oligosaccharide fragments produced were richer,and the ability in enhancing the density of zebrafish immune cells was more significant.A total of 42 acid hydrolysis degradation products were identified in the sample with the richest oligosaccharide fragments,including 8 monosaccharides,13 uronic acids,and 21 oligosaccharides(degree of polymerization 2-5).Conclusion:The acid hydrolysis degradation of structurally complex polysaccharides and the determination of corresponding immunoregulatory activities provide a basis for exploring the structure-activity relationship of polysaccharides.

关 键 词：防风多糖 酸水解 寡糖 免疫调节活性 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]