转录组测序分析沙门氏菌感染对番鸭脾脏功能的影响  

The Effect of Salmonella Infection on Spleen Function in Muscovy Ducks Based on Transcriptome Sequencing Analysis

作　　者：陶志云[1] 朱春红[1] 徐文娟[1] 宋卫涛[1] 刘宏祥[1] 王逸飞 章双杰[1] 王志成 李慧芳[1] TAO Zhiyun;ZHU Chunhong;XU Wenjuan;SONG Weitao;LIU Hongxiang;WANG Yifei;ZHANG Shuangjie;WANG Zhicheng;LI Huifang(Jiangsu Institute of Poultry Science,Yangzhou 225125,China)

机构地区：[1]江苏省家禽科学研究所,江苏扬州225125

出　　处：《华北农学报》2024年第S1期337-343,共7页Acta Agriculturae Boreali-Sinica

基　　金：江苏省种业振兴揭榜挂帅项目(JBGS(2021)111);江苏省现代农业(水禽)产业技术体系(JATS[2023]365);国家家养动物种质资源库(2024)。

摘　　要：为探讨沙门氏菌感染对番鸭的影响,拟通过转录组测序技术检测并分析沙门氏菌人工感染番鸭后不同时间脾脏组织的转录调控变化。14 d雏番鸭在肠炎沙门氏菌人工感染后1,3,10 d分别采集试验组和对照组脾脏组织样本。Illumina测序平台测序后进行生物信息学分析,筛选差异基因、信号通路,并预测基因功能。结果表明,在番鸭感染沙门氏菌1,3,10 d分别发现95,53和12个差异基因(P<0.05,|log 2(fold change)|>1)。GO分析表明,3个时间点中这些差异基因在生物学进程、分子功能、细胞部分均有富集。KEGG分析表明,感染1 d差异基因富集在细胞因子-细胞因子相互作用信号通路最多,包括CXCR4、CCL3、GDF9和CNTFR;感染3 d,富集在神经活性配体-受体相互作用信号通路的差异基因最多,包括ADRA1A、ADRA1b、GRIA1和MC5R;感染10 d获得的差异基因H2-EB1参与多条信号通路。结果提示,在番鸭沙门氏菌感染过程中这些信号通路和差异基因可能发挥了重要作用。The transcriptional regulation of spleen tissue at different time points after Salmonella infection in Muscovy ducks was analyzed by transcriptome sequencing technology,to investigate the impact of Salmonella infection on Muscovy ducks.Fourteen-day-old Muscovy ducks were infected with Salmonella enteritidis.After 1,3,and 10 days of infection,spleen tissue samples were collected from the experimental and control groups,and RNA was extracted.The Illumina sequencing platform was used for transcriptome sequencing,and changes in transcriptome,signaling pathways,and functions at different time points were identified through bioinformatics analysis.The results showed that 95,53,and 12 differential genes(P<0.05,|log 2(fold change)|>1)were found in Muscovy ducks infected with Salmonella for 1,3 and 10 days,respectively.GO analysis showed that these differential genes were enriched in biological processes,molecular functions,and cellular components at the three time points.KEGG analysis showed that at 1 day of infection,the most differentially expressed genes of CXCR4,CCL3,GDF9 and CNTFR were enriched in the cytokine-cytokine interaction signaling pathway.During the third day of infection,the differentially expressed genes of ADRA1A,ADRA1b,GRIA1,and MC5R were enriched in the neural active ligand receptor interaction signaling pathway;after 10 days of infection,the differentially expressed gene H2-EB1 participates in multiple signaling pathways,indicating that these signaling pathways and differentially expressed genes may play an important role in the infection process of Salmonella in Muscovy ducks.

关 键 词：番鸭 脾脏 转录组 沙门氏菌 

分 类 号：S834[农业科学—畜牧学]