基于宏基因组测序分析桉树林改种麻竹林对土壤和根系微生物群落的影响  

Effects of Converting Eucalypt Plantations to Dendrocalamus latiflorus Forests on Soil and Root Microbial Communities Based on Metagenomic Sequencing

作　　者：梁碧霞 田湘[1,2] 吴庆标 Liang Bixia;Tian Xiang;Wu Qingbiao(College of Forestry,Guangxi University,Nanning,Guangxi 530004,China;Liangfengjiang National Forest Park,Nanning,Guangxi 530031,China)

机构地区：[1]广西大学林学院,广西南宁530004 [2]南宁良凤江国家森林公园,广西南宁530031

出　　处：《广西林业科学》2024年第6期754-762,共9页Guangxi Forestry Science

基　　金：广西林业科技项目(桂林科研[2022ZC]第74号)。

摘　　要：为揭示桉树(Eucalyptus spp.)林改种麻竹(Dendrocalamus latiflorus)林后土壤和细根微生物群落的变化及给麻竹林可持续经营提供理论依据,以南宁市市郊饮用水水源地的麻竹林和桉树林为研究对象,分析麻竹和桉树细根及麻竹林和桉树林土壤化学性质和碳(C)、氮(N)和磷(P)化学计量比,并采用宏基因组测序技术,分析麻竹林和桉树林土壤及麻竹和桉树细根微生物群落结构和相对丰度。结果表明,桉树林改种麻竹林后,麻竹细根全碳含量、C∶N和C∶P均低于桉树细根,全磷、全氮和全钾含量及N∶P均高于桉树细根。麻竹林土壤pH值、全碳和水解性氮含量及C∶N、C∶P和N∶P均高于桉树林土壤,全磷、全氮、全钾和有效磷含量均低于桉树林土壤。麻竹林土壤、麻竹细根、桉树林土壤和桉树细根的微生物群落优势菌门均为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和酸杆菌门(Acidobacteria)。放线菌门和变形菌门相对丰度与土壤pH值和全磷含量均呈显著相关,酸杆菌门相对丰度与土壤pH值、C∶N和水解性氮含量均呈显著相关。桉树和麻竹林细根中,放线菌门相对丰度与全碳含量和C∶N均呈显著负相关,变形菌门相对丰度与C∶N呈显著正相关,酸杆菌门相对丰度与全钾含量呈显著负相关。冗余分析表明,对微生物群落结构影响较强的环境因子是全碳和全磷含量及土壤pH值。经营麻竹纯林时应该注意碳和磷的输入,加强对土壤pH值的调控。To reveal changes in microbial communities of soils and fine roots after conversion of eucalypt plantations to Dendrocalamus latiflorus forests and to provide theoretical basis for sustainable management of D.latiflorus forests,taking D.latiflorus forests and eucalypt plantations located in drinking water source areas of suburbs of Nanning as research objects,chemical properties in fine roots of D.latiflorus and eucalypt and in soils of D.latiflorus forests and eucalypt plantations as well as carbon(C),nitrogen(N),and phosphorus(P)stoichiometric ratios were analyzed.Using metagenomic sequencing technology,structures and relative abundances of microbial communities were analyzed in soils of D.latiflorus forests and eucalypt plantations and in fine roots of D.latiflorus and eucalypt.Results showed that after converting eucalypt plantations to D.latiflorus forests,total carbon content,C∶N and C∶P in fine roots of D.latiflorus were lower than those in fine roots of eucalypt,while contents of total phosphorus,total nitrogen,total potassium and N∶P in fine roots of D.latiflorus were higher than those in fine roots of eucalypt.pH,contents of total carbon and hydrolyzable nitrogen as well as C∶N,C∶P and N∶P in soils of D.latiflorus forests were higher than those in soils of eucalypt plantations,while contents of total phosphorus,total nitrogen,total potassium and available phosphorus in soils of D.latiflorus forests were lower than those in soils of eucalypt plantations.Dominant microbial phyla in soils of D.latiflorus forests and eucalypt plantations and in fine roots of D.latiflorus and eucalypt were Proteobacteria,Actinobacteria and Acidobacteria.Relative abundances of Actinobacteria and Proteobacteria had significant correlations with soil pH and total phosphorus content,while relative abundance of Acidobacteria had significant correlations with soil pH,C∶N and hydrolyzable nitrogen content.In fine roots of eucalypt and D.latiflorus,relative abundance of Actinobacteria had significantly negative correla

关 键 词：微生物群落 化学性质 宏基因组测序 优势菌门 

分 类 号：X123[环境科学与工程—环境科学]