中温、中性pH条件下污泥减量产酶菌的筛选和鉴定  

Selection and Identifi cation of Sludge Reduction Enzyme-producing Microorganisms Under Mesophilic and Neutral pH Conditions

作　　者：黄佳乐 张玉[1] Huang Jiale;Zhang Yu(School of Environmental Science and Technology,Dalian University of Technology,Liaoning,116024)

机构地区：[1]大连理工大学环境学院,辽宁116024

出　　处：《当代化工研究》2025年第1期112-114,共3页Modern Chemical Research

基　　金：中央高校基本科研业务费资助项目“石化和生活污水低温处理过程生物强化及功能微生物稳定性调控”(项目编号:DUT22LAB131)。

摘　　要：产酶菌原位污泥减量技术因其经济、节能和生态友好性的优点,逐渐得到广泛研究。目前大多数实验构建的产酶菌主要适用高温(50~80℃)或碱性(pH=9~11)的极端环境条件,这些菌株在应用于污水处理生物反应器的原位活性污泥减量时存在一定的限制。基于此,筛选出三株具有污泥减量功能的产酶菌株,其中两株产淀粉酶菌株,分别为Bacillus thuringiensis DF1(DF1)和Klebsiella huaxiensis DF10(DF10),一株产蛋白酶菌株Chryseobacterium ureilyticum DB8(DB8),三株菌的污泥减量率分别为11.35%、9.72%和11.35%。The in-situ sludge reduction technology using enzyme-producing microorganisms has gradually gained widespread research attention due to its economic,energy-saving,and eco-friendly advantages.Currently,most experimentally constructed enzyme-producing strains are primarily applicable to extreme environmental conditions such as high temperatures(50~80℃)or alkaline(pH=9~11).These strains have certain limitations when applied to in situ activated sludge reduction in wastewater treatment bioreactors.Based on this,three enzyme-producing bacterial strains with sludge reduction capabilities were identified.Two of them are amylase-producing strains,namely Bacillus thuringiensis DF1(DF1)and Klebsiella huaxiensis DF10(DF10),and one is a protease-producing strain,Chryseobacterium ureilyticum DB8(DB8).The sludge reduction rates achieved by the three strains are as follows:11.35%for DF1,9.72%for DF10,and 11.35%for DB8.

关 键 词：蛋白酶 淀粉酶 污泥减量 EPS 

分 类 号：X172[环境科学与工程—环境科学]