茶树叶中茶多酚的提取工艺优化及结构研究  

Extraction Optimization and Structural Characterization of Tea Polyphenols from Camellia Sinensis Leaves

作　　者：杨宇蕊 邓佺辛 吴彤月 孙菲菲 罗倩 郭林新 Yang Yurui;Deng Quanxin;Wu Tongyue;Sun Feifei;Luo Qian;Guo Linxin(The Youth Innovation Team of Shaanxi Universities of Development and Utilization of Qinba Chinese Medicine Resources,Ankang University,Shaanxi,725000;Qinling-Bashan Mountains Bioresources Comprehensive Development Collaborative Innovation Center,Shaanxi University of Technology,Shaanxi,723001)

机构地区：[1]秦巴中药资源开发与应用陕西高校青年创新团队安康学院,陕西725000 [2]陕南秦巴山区生物资源综合开发协同创新中心陕西理工大学,陕西723001

出　　处：《当代化工研究》2025年第1期170-172,共3页Modern Chemical Research

基　　金：陕西省教育厅重点科学研究计划协同创新项目(项目编号:23JY019);陕西省大学生创新创业训练计划项目(项目编号:S202211397054);安康市科技计划项目(项目编号:AK2023-NY-26);安康市茶叶功能性成分研发应用工程技术研究中心建设项目(项目编号:2024AYHX070)。

摘　　要：为对茶树叶中茶多酚的提取及结构进行研究,采用单因素及正交实验设计,优化提取条件,并鉴别提取物的结构。实验结果表明,在乙醇体积分数为70%,提取温度60℃,提取时间100 min,液料比60 mL/g的工艺条件下,茶树叶中茶多酚的提取得率可达243 mg/g。进一步采用红外光谱,检测到3276 cm-1处酚羟基伸缩振动及1686 cm-1处羰基伸缩振动等信号峰,从而对茶多酚的结构进行了鉴别。In order to explore the extraction and structure of tea polyphenols from camellia sinensis leaves,single factor and orthogonal ex-perimental design were used to optimize the extraction conditions,and identify the structure of the extract.The experimental results showed that the extraction yield of polyphenols from leaves could reach 243 mg/g under the process conditions of 70%ethanol concentration,extraction temperature of 60℃,extraction time of 100 min,and liquid/material ratio of 60 mL/g.Furthermore,the phenolic hydroxyl stretching vibration at 3276 cm-1 and the carbonyl stretching vibration at 1686 cm-1 were detected by FTIR to identify the structure of tea polyphenols.

关 键 词：茶树叶 多酚 提取工艺 红外光谱 

分 类 号：TQ072[化学工程]