Garvicin LG6对不同溶血表型金黄色葡萄球菌的抗菌活性  

Antimicrobial activity of garvicin LG6 against Staphylococcus aureus of different hemolytic phenotypes

作　　者：唐伟[1,2] 翁胜男 王亚武 姚杰 李昕[2] 周强[2] 徐元宏[1] TANG Wei;WENG Shengnan;WANG Yawu;YAO Jie;LI Xin;ZHOU Qiang;XU Yuanhong(Department of Laboratory Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China;Department of Laboratory Medicine,The Se-cond Hospital of Anhui Medical University,Hefei 230601,China)

机构地区：[1]安徽医科大学第一附属医院检验科,安徽合肥230022 [2]安徽医科大学第二附属医院检验科,安徽合肥230601

出　　处：《中国感染控制杂志》2025年第1期23-29,共7页Chinese Journal of Infection Control

基　　金：国家自然科学基金项目(82102460);安徽医科大学第二附属医院临床研究培育计划项目(2021LCZD13);安徽医科大学校科研基金项目(2022xkj197)。

摘　　要：目的探索格氏乳球菌SHAMU-LG6所分泌的细菌素(初步命名为garvicin LG6)对不同溶血表型金黄色葡萄球菌的抗菌活性。方法收集分离自2021—2023年安徽某医院临床患者的金黄色葡萄球菌,三点接种法检测金黄色葡萄球菌的溶血表型,并根据药敏检测结果将各溶血表型金黄色葡萄球菌进一步划分为甲氧西林敏感金黄色葡萄球菌(MSSA)和耐甲氧西林金黄色葡萄球菌(MRSA)。牛津杯法抑菌试验检测格氏乳球菌SHAMU-LG6对不同溶血表型金黄色葡萄球菌的拮抗活性。对格氏乳球菌SHAMU-LG6进行全基因组测序,使用antiSMASH 7.0和BAGEL4在线数据库,寻找garvicin LG6的生物合成基因簇。经过大孔树脂吸附、梯度乙醇洗脱、旋转蒸发干燥和旋干物复溶,牛津杯法抑菌试验检测garvicin LG6粗提物对金黄色葡萄球菌的抗菌活性。结果格氏乳球菌SHAMU-LG6能够明显拮抗不同溶血表型的MSSA和MRSA。格氏乳球菌SHAMU-LG6的染色体基因组上存在garvicin LG6生物合成基因簇。格氏乳球菌SHAMU-LG6单个菌落或6 mL发酵液所分泌的garvicin LG6抗菌活性至少相当于30μg头孢西丁药敏纸片。结论Garvicin LG6能够高效杀伤不同溶血表型MSSA和MRSA,具有开发成新型抗菌药物的潜力,临床应用前景很大。Objective To explore the antimicrobial activity of the bacteriocin(tentatively named garvicin LG6)secreted by Lactococcus garvieae(L.garvieae)SHAMU-LG6 against Staphylococcus aureus(S.aureus)of different hemolytic phenotypes.Methods S.aureus isolated from clinical patients in a hospital of Anhui from 2021 to 2023 were collected.The hemolytic phenotypes of S.aureus were detected by three-point inoculation method.S.aureus of different hemolytic phenotypes were further categorized into methicillin-sensitive S.aureus(MSSA)and methicillin-resistant S.aureus(MRSA)according to antimicrobial susceptibility testing results.The antagonistic activity of L.garvieae SHAMU-LG6 against S.aureus of different hemolytic phenotypes was assayed by Oxford cup me-thod.The whole-genome sequencing of L.garvieae SHAMU-LG6 was performed.Biosynthetic gene cluster of garvicin LG6 was searched by online databases antiSMASH 7.0 and BAGEL4.Through macroporous resin adsorption,ethanol gradient elution,rotary evaporation,and dried material reconstitution,antimicrobial activity of garvicin LG6 crude extract against S.aureus was detected by the inhibitory testing of Oxford cup method.Results L.garvieae SHAMU-LG6 could significantly antagonize MSSA and MRSA of different hemolytic phenotypes.Biosynthetic gene cluster of garvicin LG6 was present on the chromosomal genome of L.garvieae SHAMU-LG6.The antimicrobial activity of garvicin LG6 secreted by a single colony or 6 mL fermentation fluid of L.garvieae SHAMU-LG6 were at least equal to that of antibiotic disc of 30μg cefoxitin.Conclusion Garvicin LG6 can efficiently kill MSSA and MRSA of different hemolytic phenotypes,and has the potential to be developed into a novel antimicrobial agent,which has great prospects for clinical application.

关 键 词：格氏乳球菌 细菌素 金黄色葡萄球菌 溶血表型 抗菌活性 

分 类 号：R378.11[医药卫生—病原生物学]