抗坏血酸引发对Na2SO4胁迫下饲用小黑麦种子活力的影响  

Effects of Ascorbic Acid Priming on Seed Vigor of the Forage Triticale under Na2SO4 Stress

作　　者：郭嘉庆 夏方山 李韩晶 白朝瑞 张金成 李嘉俊 GUO Jiaqing;XIA Fangshan;LI Hanjing;BAI Chaorui;ZHANG Jincheng;LI Jiajun(College of Grassland Science,Shanxi Agricultural University,Taigu030801,China)

机构地区：[1]山西农业大学草业学院,山西太谷030801

出　　处：《畜牧与饲料科学》2024年第6期75-80,共6页Animal Husbandry and Feed Science

基　　金：山西省重点研发计划项目(202102140601006);山西农业大学“十四五”生物育种工程项目(YZGC134);内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题(2021KF0303)。

摘　　要：[目的]探究Na2SO4胁迫下外源抗坏血酸(ascorbic acid,AsA)引发对饲用小黑麦种子活力的影响。[方法]分别以浓度为0.5、1.0、1.5、2.0 mmol/L的AsA溶液引发处理0(CK)、4、8、16、24 h后,在浓度为75 mmol/L的Na2SO4溶液胁迫下进行饲用小黑麦种子发芽试验,对发芽率(germination percentage,Gp)、发芽指数(germination index,Gi)、幼苗活力指数(seedlings vigor index,SVI)、平均发芽时间(mean germination time,MGT)的变化规律进行分析。[结果]AsA浓度对饲用小黑麦种子Gp、Gi和SVI的影响极显著(P<0.01),对MGT的影响不显著(P>0.05);AsA引发时间对饲用小黑麦种子Gp、Gi、MGT和SVI的影响极显著(P<0.01);AsA浓度与引发时间之间的相互作用对饲用小黑麦种子Gp和SVI的影响极显著(P<0.01),对Gi和MGT的影响不显著(P>0.05)。AsA浓度为1.5 mmol/L引发8 h时,饲用小黑麦种子的Gp、Gi和SVI均最高,MGT最短;AsA浓度为2.0 mmol/L引发24 h时,饲用小黑麦种子的Gp、Gi和SVI均低于其他引发时间,而MGT长于其他引发时间。[结论]外源AsA引发对Na2SO4胁迫下饲用小黑麦种子活力的影响,与其浓度、引发时间及两者的交互作用密切相关。以浓度为1.5 mmol/L的AsA处理8 h对75 mmol/L Na2SO4胁迫下饲用小黑麦种子活力的引发效果最好。[Objective]The aim of this study was to assess the effects of exogenous ascorbic acid(AsA)priming on seed vigor of forage triticale under Na2SO4 stress.[Method]The seed germination test of forage triticale was carried out under the stress of Na2SO4 solution at the concentration of 75 mmol/L after the treatment of 0(CK),4,8,16,and 24 h with AsA solution at the concentrations of 0.5,1.0,1.5,and 2.0 mmol/L.Subsequently,the changes of germination percentage(Gp),germination index(Gi),seedlings vigor index(SVI),and mean germination time(MGT)of the seeds were analyzed.[Result]The effects of AsA concentration on Gp,Gi and SVI of forage triticale seeds were highly significant(P<0.01),and the effects on MGT were not significant(P>0.05).The effects of AsA priming time on Gp,Gi,MGT and SVI of forage triticale seeds was highly significant(P<0.01).The interaction between AsA concentration and priming time was highly significant(P<0.01)for Gp and SVI and non-significant(P>0.05)for Gi and MGT of forage triticale seeds.The AsA concentration of 1.5 mmol/L triggered for 8 h resulted in the highest Gp,Gi and SVI as well as the shortest MGT in forage triticale seeds.When AsA concentration was 2.0 mmol/L for 24 h,Gp,Gi and SVI of forage triticale seeds were lower than other priming times,while MGT was longer than other priming times.[Conclusion]The effects of exogenous AsA priming on seed vigor of forage triticale under Na2SO4 stress were closely related to its concentration,priming time and the interaction between the two.AsA at a concentration of 1.5 mmol/L for 8 h possessed the best effect on the seed vigor of forage triticale under 75 mmol/L Na2SO4 stress.

关 键 词：饲用小黑麦 NA2SO4胁迫 抗坏血酸 种子引发 种子活力 

分 类 号：S544.904.1[农业科学—作物学]