酵母菌及其与芽孢杆菌复配去除水泡粪污水氨氮效果的研究  

Research of Ammonia Nitrogen Removal Effects by Yeasts and Their Combination with Bacillus spp.on Effluent from Pig Manure Cleaning by Water Submerging

作　　者：丁佳乐 韩先杰[2] 李树文[1] DING Jiale;HAN Xianjie;LI Shuwen(College of Life Science,Qingdao Agricultural University,Shandong Province Key Laboratory of Applied Mycology,Qingdao266109,China;College of Veterinary Medicine,Qingdao Agricultural University,Qingdao266109,China)

机构地区：[1]青岛农业大学生命科学学院/山东省应用真菌重点实验室,山东青岛266109 [2]青岛农业大学动物医学院,山东青岛266109

出　　处：《畜牧与饲料科学》2024年第6期113-121,共9页Animal Husbandry and Feed Science

基　　金：山东省现代农业产业技术体系生猪生产与环境控制岗位项目(SDAIT-08-09)。

摘　　要：[目的]考察4株酵母菌株及其与芽孢细菌组合对水泡粪污水的脱氨氮效果。[方法]以氨氮去除率和氨气去除率为测量指标,研究酿酒酵母ZP (Saccharomyces cerevisiae ZP)、酿酒酵母X1(Sac.cerevisiae X1)、乙醇假丝酵母JMJ(Candida ethanolica JMJ)、乙醇假丝酵母HJ(Can. ethanolica HJ)4株酵母菌株单独以1%的量接种对水泡粪污水的脱氨氮效果;将乙醇假丝酵母JMJ株与巨大芽孢杆菌LB株和地衣芽孢杆菌WL株、乙醇假丝酵母HJ株与巨大芽孢杆菌LB株和地衣芽孢杆菌WL株分别以1.5%的接种量组合接种对水泡粪污水的脱氨氮效果,找出酵母菌与芽孢杆菌的最优组合;将乙醇假丝酵母JMJ株和巨大芽孢杆菌LB株分别以0.5%、1%、1.5%的接种量组合接种对水泡粪污水的脱氨氮效果,找出酵母菌与芽孢杆菌的最优组合比例。[结果]4株酵母菌株单独处理污水时都具有去除氨气的能力,在接种后6~72 h氨气相对去除率范围是17.90%~75.41%,氨氮相对去除率较低,且氨氮含量变化不稳定;酵母菌株与芽孢杆菌复配时,乙醇假丝酵母JMJ和巨大芽孢杆菌LB组合氨气和氨氮去除效果较好,且具有长效性,氨气相对去除率在3~6 h维持在38.53%~41.45%,12~72 h达到100.00%,氨氮相对去除率在24~72 h维持在17.29%~25.65%,在去除效率和稳定性方面1.50%的接种量都优于0.50%和1.00%。[结论]酵母和芽孢菌组合可用于水泡粪污水氨氮处理,且组合比单独酵母去除氨氮效率高,研究结果为水泡粪污水氨氮的有效处理和资源化利用提供了理论指导和实践参考。[Objective]The deamination and nitrogen removal effects of four yeast strains and their combinations with spore bacteria on the wastewater from blister manure were investigated.[Method]Taking the removal rates of ammonia nitrogen and ammonia gas as measurement indicators,this study investigated the deamination effect on piggery wastewater containing blisters when inoculated with four yeast strains:Saccharomyces cerevisiae ZP,Sac.cerevisiae X1,Candida ethanolica JMJ,and Can.ethanolica HJ,at a dosage of 1%.The deamination and nitrogen removal efficiencies of combined inoculations involving Candida ethanolica strains JMJ and HJ with Bacillus megaterium strain LB and Bacillus licheniformis strain WL,at an inoculation concentration of 1.5%,were evaluated using wastewater from blister manure as the medium.This study aimed to determine the optimal combination of yeast and bacillus strains for effective wastewater treatment.The deamination and nitrogen removal efficacy of co-inoculating Candida ethanolica strain JMJ and Bacillus megaterium strain LB at inoculation concentrations of 0.5%,1%,and 1.5%in wastewater from blister manure was investigated to determine the optimal combination ratio of yeast and bacillus.[Result]When the four yeast strains were individually employed to treat sewage,all demonstrated the capability to remove ammonia.The relative removal rate of ammonia ranged from 17.90%to 75.41%within 6 to 72 hours post-inoculation.When yeast strains are co-cultured with bacillus,the combination of Candida ethanolica JMJ and Bacillus megaterium LB demonstrates superior performance in the removal of ammonia and ammonia nitrogen,exhibiting long-term effectiveness.The relative removal rate of ammonia gas remains at 38.53%-41.45%within the initial 3 to 6 hours and reaches 100.00%within 12 to 72 hours.The relative removal rate of ammonia nitrogen is maintained between 17.29%and 25.65%over a period of 24 to 72 hours.In terms of both removal efficiency and stability,an inoculation amount of 1.50%outperforms those of 0.50%

关 键 词：水泡粪污水 氨氮 酵母 芽孢杆菌 去除 

分 类 号：X703.5[环境科学与工程—环境工程] X713