宫颈癌患者血清LncRNA SFTA1P和LncRNA UBE2R2-AS1表达水平与预后的关系  

Relationship between serumexpression levels of LncRNA SFTA1P and LncRNA UBE2R2-AS1 with prognosis in cervical can⁃cer patients

作　　者：肖献花 郭丽娜[1] 呼慧军 张伟伟 冀娜娜 段敬梅 高翔[2] XIAO Xianhua;GUO Lina;HU Huijun;ZHANG Weiwei;JI Nana;DUAN Jingmei;GAO Xiang(Department of Gynecology Handan Maternal and Child Health Hospital,Handan 056001,China;Department of Gynecology Handan First Hospital,Handan 056001,China)

机构地区：[1]邯郸市妇幼保健院妇科,河北邯郸056001 [2]邯郸市第一医院妇科,河北邯郸056001

出　　处：《安徽医学》2025年第1期22-27,共6页Anhui Medical Journal

基　　金：河北省邯郸市科学技术研究与发展计划项目(编号:1622201051-2)。

摘　　要：目的探究宫颈癌(CC)患者血清中长链非编码RNA(LncRNA)表面活性剂相关1假基因(SFTA1P)和UBE2R2-AS1表达水平及其与预后的关系。方法选取2018年4月至2021年4月邯郸市妇幼保健院收治的108例CC患者为CC组,100例同期健康体检者为健康组。用实时荧光定量PCR(qRT-PCR)法检测血清LncRNA SFTA1P和LncRNA UBE2R2-AS1水平;绘制受试者工作特征(ROC)曲线评估血清LncRNA SFTA1P和LncRNA UBE2R2-AS1对CC的诊断价值;Kaplan-Meier法分析血清LncRNA SFTA1P和LncRNA UBE2R2-AS1表达水平与CC患者预后的关系;Cox回归分析CC患者预后的影响因素。结果与健康组相比,CC患者血清LncRNA SFTA1P表达水平升高(P<0.05),LncRNA UBE2R2-AS1表达水平降低(P<0.05),且其水平与FIGO分期、淋巴结转移、肌层浸润深度有关(P<0.05);血清LncRNA SFTA1P和LncRNA UBE2R2-AS1单独及联合诊断CC发生的曲线下面积分别为0.863、0.817和0.915,联合优于单独诊断(Z_(二者联合-LncRNA SFTA1P)=3.057、P=0.002,Z_(二者联合-LncRNA UBE2R2-AS1)=3.564、P<0.001);血清LncRNA SFTA1P高表达组患者3年生存率低于低表达组患者(64.91%比80.39%,χ^(2)=4.443,P=0.035),LncRNA UBE2R2-AS1高表达组患者3年生存率高于低表达组患者(82.00%比63.79%,χ^(2)=5.938,P=0.015);FIGO分期Ⅱb~Ⅲc期、淋巴结转移、肌层浸润深度≥1/2、LncRNA SFTA1P表达水平升高及LncRNA UBE2R2-AS1表达水平降低均为CC患者预后的危险因素(P<0.05)。结论CC患者血清中LncRNA SFTA1P表达上调,LncRNA UBE2R2-AS1表达下调,其表达水平与肿瘤FIGO分期、淋巴结转移、肌层浸润深度有关,且与患者预后生存密切相关。Objective To investigate the expression levels of long non-coding RNA(LncRNA)surfactant associated 1 pseudogene(SFTA1P)and ubiquitin-conjugating enzyme E2R2 antisense RNA 1(UBE2R2-AS1)in the serum of cervical cancer(CC)patients and their correlation with prognosis Methods A total of 108 CC patients admitted to Handan Maternal and Child Health Hospital between April 2018 and April 2021 were selected as the CC group,and 100 healthy individuals during the same period were selected as the health group.Real time fluorescence quantitative PCR(qRT-PCR)was used to detect the levels of serum LncRNA SFTA1P and LncRNA UBE2R2-AS1 in each group;receiver operating characteristic(ROC)curve was plotted to evaluate the diagnostic value of serum LncRNA SFTA1P and LncRNA UBE2R2-AS1 for CC;Kaplan-Meier method was used to analyze the relationship between the expression levels of serum LncRNA SFTA1P and LncRNA UBE2R2-AS1 and the prognosis of CC patients;Cox regression was applied to analyze the influencing factors of prognosis in CC patients.Re-sults Compared with the health group,the expression level of serum LncRNA SFTA1P in CC patients increased(P<0.05),the expression level of LncRNA UBE2R2-AS1 was reduced(P<0.05),and their expression levels were related to FIGO staging,lymph node metastasis,and depth of muscle layer infiltration(P<0.05).The area under the curve for diagnosing CC by serum LncRNA SFTA1P and LncRNA UBE2R2-AS1 alone and in combination was 0.863,0.817,and 0.915,respectively,and the combined diagnosis was superior to individual diagnoses(Z_(com-bination-LncRNASFTA1P)=3.057,P=0.002,Z_(combination-LncRNAUBE2R2-AS1)=3.564,P<0.001).The 3-year survival rate in the serum LncRNA SFTA1P high ex-pression group was lower than that in the low expression group(64.91%vs 80.39%,χ^(2)=4.443,P=0.035),and the 3-year survival rate in the Ln-cRNA UBE2R2-AS1 high expression group was higher than that in the low expression group(82.00%vs 63.79%,χ^(2)=5.938,P=0.015).FIGO staging of stages IIb~IIIc,lymph node metastasis,muscle layer infiltr

关 键 词：宫颈癌 长链非编码RNA SFTA1P 长链非编码RNA UBE2R2-AS1 临床病理特征 预后 

分 类 号：R737.33[医药卫生—肿瘤]