miR-96对膀胱癌T24细胞增殖及迁移的影响  

The impact of miR-96on the proliferation and migration of bladder cancer T24cells

作　　者：赵兴东 黄德斌 王俊凯 谷欣权[1] ZHAO Xingdong;HUANG Debin;WANG Junkai;GU Xinquan(Ward 1 of the Urology Department,China-Japan Union Hospital of Jilin University,Changchun 130033,China)

机构地区：[1]吉林大学中日联谊医院泌尿外科一病区,吉林长春130033

出　　处：《中国实验诊断学》2025年第1期79-84,共6页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省财政厅项目(ZXSY2023011)。

摘　　要：目的探讨miR-96对膀胱癌T24细胞的生长、增殖、迁移和凋亡的影响。方法本研究选取膀胱癌T24细胞作为实验对象,通过细胞培养及转染的方式将miR-96抑制剂片段引入T24细胞内,培养48h后。透过荧光镜及光学显微镜观察对比,细胞的转染效率达到80%以上方可进行后续的实验。后续应用CCK-8法、流式细胞术(Flow CytoMetry,FCM)、划痕实验(wound healing)和免疫印迹(Western Blot,WB)分析了T24细胞的生长状况、凋亡情况、侵袭能力以及EPB41L3蛋白表达情况。结果在miR-96抑制剂片段转染后的T24细胞的迁移能力减弱,增殖速度减缓,凋亡率升高。结论miR-96能够促进T24细胞的生长、增殖及侵袭,抑制T24细胞的凋亡。Objective The aim of this study was to investigate the effects of miR-96on the growth,proliferation,migration,and apoptosis of bladder cancer T24cells.Methods This study selected T24bladder cancer cells as the experimental subjects.Using cell culture and transfection methods,the miR-96inhibitor fragment was introduced into T24 cells,and after 48hours of culture,the transfection efficiency was assessed through fluorescence microscopy and optical microscopy.A transfection efficiency exceeding 80%was deemed adequate for subsequent experiments.Following this,the CCK-8assay,flow cytometry(FCM),wound healing assay,and Western blot(WB)were employed to analyze the growth status,apoptosis,invasive capabilities,and expression levels of the EPB41L3protein in T24cells.Results After transfection with miR-96inhibitor fragments,the migration ability of T24cells decreased,the growth rate slowed,and the apoptosis rate increased.Conclusion miR-96can promote the growth and invasion of T24cells,while inhibiting the apoptosis of T24cells.

关 键 词：膀胱癌 miR-96 T24细胞 细胞增殖 细胞迁移 

分 类 号：R73[医药卫生—肿瘤]