食管鳞癌组织中IRF4、MDM2、C-myc表达与预后的关系研究  

Relationship between IRF4,MDM2,C⁃myc and Prognosis in Esophageal Squamous Cell Carcinoma

作　　者：江汝伟 黄思宇 JIANG Ruwei;HUANG Siyu(Department of Medical Oncology,Yingshang County People's Hospital,Yingshang,Anhui 236200,China;Department of Oncology,Fuyang Second People's Hospital,Fuyang,Anhui 236015,China)

机构地区：[1]安徽省颍上县人民医院肿瘤内科,安徽阜阳236200 [2]阜阳市第二人医院肿瘤科,安徽阜阳236015

出　　处：《转化医学杂志》2024年第7期1005-1009,共5页Translational Medicine Journal

基　　金：2021阜阳市卫生健康委科研项目(FY2021-171)。

摘　　要：目的探讨食管鳞状细胞癌(ESCC)组织中干扰素调节因子4(IRF4)、小鼠双微体基因2(MDM2)、核转录因子C-myc表达与预后的关系。方法收集2020年1月至2023年6月在安徽省颍上县人民医院进行治疗的65例ESCC患者癌组织标本为研究组,并收集同期30例食道良性病变患者食道组织标本为对照组,免疫组化法检测组织中IRF4、MDM2、C-myc表达情况,比较不同组织间IRF4、MDM2、C-myc表达差异。并收集ESCC患者临床肿瘤特征资料,分析各指标表达与肿瘤特征的关系,比较不同IRF4、MDM2、C-myc表达水平患者的术后生存差异,分析影响ESCC患者预后的危险因素。结果ESCC癌组织IRF4、MDM2、C-myc阳性表达率高于良性病变组织,差异有统计学意义(P<0.05)。IRF4阳性表达与侵犯外膜有无、TNM分期有关(P<0.05);MDM2阳性表达与分化程度、淋巴转移有无、TNM分期有关(P<0.05);C-myc阳性表达与分化程度、侵犯外膜有无、TNM分期有关(P<0.05)。IRF4、MDM2、C-myc阳性表达患者总生存率为47.62%(10/21)、50.00%(17/34)、58.14%(25/43),均低于阴性表达患者的79.55%(35/44)、90.32%(28/31)、90.91%(20/22)(Log rankχ2=10.100、13.700、7.526,P<0.05)。多因素Cox模型分析结果显示,TNM分期(Ⅲ+Ⅳ期)、IRF4、MDM2、C-myc阳性为影响ESCC患者预后的独立危险因素(P<0.05)。结论IRF4、MDM2、C-myc在ESCC癌组织中阳性表达率高,与肿瘤发生发展有关,是影响患者预后的独立因素。Objective To explore the relationship between interferon regulatory factor 4(IRF4),murine double mi⁃nute 2(MDM2),nuclear transcription factor C⁃myc and prognosis in esophageal squamous cell carcinoma(ESCC).Methods The samples of cancer tissues were collected from 65 patients with ESCC who were treated in the Yingshang county people's hospital between January 2020 and June 2023,while samples of normal esophageal tissues samples from 30 patients with be⁃nign esophageal lesions were collected as controls.The expressions of IRF4,MDM2 and C⁃myc in different tissues were detec⁃ted by immunohistochemistry,and differences of the three indexes in different tissues were compared.The clinical tumor char⁃acteristics data in ESCC patients were collected to analyze the relationship between different indexes and tumor characteristics.The differences in postoperative survival among patients with different expressions of IRF4,MDM2 and C⁃myc were compared.The risk factors of prognosis in ESCC patients were analyzed.Results The positive expression rates of IRF4,MDM2 and C⁃myc in ESCC tissues were higher than those in benign lesion tissue(P<0.05).The positive expression of IRF4 was correla⁃ted with invasion of outer membrane and TNM staging(P<0.05).The positive expression of MDM2 was correlated with dif⁃ferentiation degree,lymphatic metastasis and TNM staging(P<0.05).The positive expression of C⁃myc was correlated with differentiation degree,invasion of outer membrane and TNM staging(P<0.05).The overall survival rates in patients with positive expressions of IRF4,MDM2 and C⁃myc were 47.62%(10/21),50.00%(17/34)and 58.14%(25/43),lower than those with negative expressions[(79.55%(35/44),90.32%(28/31),90.91%(20/22);Log rank χ^(2)=10.100,13.700,7.526,P<0.05)].The results of multivariate COX model analysis showed that TNM staging at stageⅢ+Ⅳ,positive IRF4,MDM2 and C⁃myc were independent risk factors of prognosis in ESCC patients(P<0.05).Conclusion The positive expression rates of IRF4,MDM2 and C⁃myc are hi

关 键 词：食管鳞状细胞癌 干扰素调节因子4 小鼠双微体基因2 核转录因子C-myc 预后 

分 类 号：R735.1[医药卫生—肿瘤]