基于高效液相色谱指纹图谱的仿野生天麻品质及抗氧化和神经保护活性  

Wild-Simulated Gastrodia elata Bl.:Quality Assessment by High Performance Liquid Chromatography Fingerprinting and Antioxidant and Neuroprotective Activities

作　　者：杨修 严艳芳 李齐激 杨礼寿 潘雄 李立郎 胡薛豪 陈瑞 罗夫来[3] 高明 杨小生 YANG Xiu;YAN Yanfang;LI Qiji;YANG Lishou;PAN Xiong;LI Lilang;HU Xuehao;CHEN Rui;LUO Fulai;GAO Ming;YANG Xiaosheng(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Natural Products Research Center of Guizhou Province,Guiyang 550025,China;College of Agriculture,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州省天然产物研究中心,贵州贵阳550025 [3]贵州大学农学院,贵州贵阳550025

出　　处：《食品科学》2025年第5期226-235,共10页Food Science

基　　金：贵州省科技计划项目(黔科合基础-ZK[2024]一般639;黔科合成果[2024]一般087);国家重点研发计划项目乡村产业专项(2021YFD1601001);贵州省教育厅高等学校特色重点实验室建设项目(黔教合KY字[2020]018号)。

摘　　要：建立仿野生天麻的高效液相色谱(high performance liquid chromatography,HPLC)指纹图谱及多成分含量测定方法,结合指纹图谱相似度评价、主成分分析和正交偏最小二乘判别分析(orthogonal partial least squaresdiscriminant analysis,OPLS-DA)探究不同产地仿野生天麻的品质差异。通过比较分析贵州省主产区15批天麻样品的HPLC标志成分指纹图谱,共标定出14个共有峰,指认天麻素、对羟基苯甲醇、巴利森苷E、巴利森苷B、巴利森苷C、巴利森苷A 6个标志成分。主成分分析表明,天麻中前3个主成分累计方差贡献率达83.373%,天麻素和巴利森苷A是影响天麻类别的主要成分;OPLS-DA明确了天麻素、对羟基苯甲醇、巴利森苷C、巴利森苷B和巴利森苷A是影响天麻质量的成分。天麻标志成分总含量为12.957~36.738 mg/g,巴利森苷A的含量占比最大。在同等限定条件下,仿野生和袋栽天麻的标志成分差异明显,利用试剂盒和体外细胞实验研究天麻的抗氧化能力和神经细胞保护活性,结果显示仿野生天麻在1,1-二苯基-2-三硝基苯肼自由基、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基、羟自由基清除能力及PC12细胞和HT-22神经细胞的保护活性方面均优于袋栽天麻。本研究可为仿野生天麻的质量标准建立提供数据支撑。High performance liquid chromatography(HPLC)fingerprints and multi-component determination methods were established to investigate the differences in quality among the dried tubers of wild-simulated Gastrodia elata Bl.(Rhizoma Gastrodiae)from different geographical origins by combining fingerprint similarity evaluation,principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA).By comparative analysis of the fingerprints of 15 batches of Rhizoma Gastrodiae samples from major production regions in Guizhou province,a total of 14 shared peaks were identified and assigned to 6 signature components,including gastrodin,p-hydroxybenzyl alcohol,parishin E,parishin B,parishin C and parishin A.PCA showed that the first three principal components cumulatively explained 83.373%of the variance,and gastrodin and parishin A were selected as markers to discriminate between different categories of Rhizoma Gastrodiae.OPLS-DA ascertained that gastrodin,p-hydroxybenzyl alcohol,parishin C,parishin B,and parishin A affected the quality of Rhizoma Gastrodiae.The total content of signature components of Rhizoma Gastrodiae was 12.957–36.738 mg/g,and parishin A accounted for the largest proportion of it.Under the same limiting condition,significant differences in the contents of signature components were observed between wild-simulated and bag-cultivated Rhizoma Gastrodiae.The antioxidant capacity and neuroprotective activity of Rhizoma Gastrodiae were investigated by kits and in vitro cellular experiments,revealing that the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cationic radical and hydroxyl radical scavenging capacity and neuroprotective activities of wild-simulated Rhizoma Gastrodiae on PC12 and HT-22 neurons were superior to those of bag-cultivated Rhizoma Gastrodiae.This study provides data support for the establishment of quality standards for wild-simulated Rhizoma Gastrodiae.

关 键 词：天麻 指纹图谱 多成分含量测定 抗氧化 神经细胞保护 

分 类 号：O657.63[理学—分析化学]