多发性骨髓瘤患者EB病毒DNA的检测分析  

DNA Detection and Analysis of EB Virus of Patients with Multiple Myeloma

作　　者：徐根娣 尹明赵 郭运丽 王媚 屠国占 XU Gendi;YIN Mingzhoa;GUO Yunli;WANG Mei;TU Guozhan(Qiannan Buyi and Miao Autonomous Prefecture People's Hospital,Xingyi,Guizhou 562400;Qiannan Branch,Guizhou Provincial Armed Police,Xingyi,Guizhou 562400)

机构地区：[1]黔西南布依族苗族自治州人民医院,贵州兴义562400 [2]贵州省武警黔西南支队,贵州兴义562400

出　　处：《智慧健康》2024年第31期69-72,共4页Smart Healthcare

摘　　要：目的 分析多发性骨髓瘤患者EB病毒DNA的检测结果。方法 回顾性选取2021年10月—2023年10月黔西南州人民医院收治的多发性骨髓瘤患者43例作为多发性骨髓瘤组,另外选取了76例有其他EB病毒相关疾病的患者作为阳性对照组,以及50名健康体检人员作为阴性对照组。采用PCR技术对EBV-DNA进行检测,统计分析三组EBV-DNA检测结果,并统计分析多发性骨髓瘤组EBV-DNA阳性患者干扰素治疗前后EBV变化。结果 阳性对照组骨髓有核细胞的EBV-DNA检测结果显著高于多发性骨髓瘤组和阴性对照组(P<0.05)。多发性骨髓瘤组的骨髓有核细胞EBV-DNA检测阳性率亦显著高于阴性对照组(P<0.05)。阳性对照组外周血淋巴细胞的EBV-DNA检测阳性率高于多发性骨髓瘤组和阴性对照组(P<0.05)。多发性骨髓瘤组的外周血淋巴细胞EBV-DNA检测阳性率亦显著高于阴性对照组(P<0.05)。此外,多发性骨髓瘤组患者的血浆EBV-DNA检测阳性率显著高于阳性对照组和阴性对照组(P<0.05)。多发性骨髓瘤组患者接受干扰素治疗后,EBV-DNA阳性患者的阳性率低于治疗前,而阴性率则高于治疗前(P<0.05)。结论 多发性骨髓瘤患者EB病毒DNA的检测阳性率高,在多发性骨髓瘤发病中,EBV可能发挥着极为重要的作用,干扰素治疗能够将多发性骨髓瘤患者的EBV-DNA阳性率降低,从而对患者病情进展进行有效抑制。Objective To analyze DNA detection results of EB virus of patients with multiple myeloma.Methods The paper reviewed and chose 43 patients with multiple myeloma from October 2021 to October 2023 in Qiannan Prefecture People's Hospital as the multiple myeloma group,and 76 patients with other EB virus related diseases as the positive control group,and 50 healthy physical examination people as the negative control group.EBV-DNA was detected with PCR technology,EBV-DNA detection results were statistically analyzed among three groups,and EBV changes of EBV-DNA positive patients with multiple myeloma were statistically analyzed before and after interferon treatment.Results EBV-DNA detection results of bone marrow nucleated cells in the positive control group were significantly higher than the multiple myeloma group and the negative control group(P<0.05).Positive rate of EBV-DNA detection in bone marrow nucleated cells in the multiple myeloma group was significantly higher than the negative control group(P<0.05).Positive rate of EBV-DNA detection in peripheral blood lymphocytes in the positive control group was higher than the multiple myeloma group and the negative control group(P<0.05).Positive rate of EBV-DNA detection in peripheral blood lymphocytes of the multiple myeloma group was significantly higher than the negative control group(P<0.05).In addition,positive rate of plasma EBV-DNA detection in the multiple myeloma group was significantly higher than the positive and negative control groups(P<0.05).In the multiple myeloma group,positivity rate of EBV-DNA positive patients after interferon treatment was lower than before treatment,while negative rate was higher than before treatment(P<0.05).Conclusion DNA detection of EBV of patients with multiple myeloma has high positivity rate,and EBV may play an extremely important role i n development of multiple myeloma,and interferon treatment can reduce positivity rate of EBV-DNA of patients with multiple myeloma,and inhibit progression of the disease effectively.

关 键 词：多发性骨髓瘤 EB病毒 DNA 

分 类 号：R73[医药卫生—肿瘤]