加味芍药甘草汤含药血清对子宫腺肌病异位子宫内膜间质细胞迁移、侵袭和血管生成的影响  

Effect of Modified Shaoyao(芍药)-Gancao(甘草)Decoction on Migration,Invasion,and Angiogenesis of Endometrial Stromal Cells in Ectopic Adenomyosis

作　　者：温小慧 刘学红 黄诗雅 李坤寅 关永格 WEN Xiaohui;LIU Xuehong;HUANG Shiya;LI Kunyin;GUAN Yongge(The Third Clinical School of Guangzhou University of Chinese Medicine,Guangzhou 510000;The Third Afiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510000)

机构地区：[1]广州中医药大学第三临床医学院,广州510000 [2]广州中医药大学第三附属医院,广州510000

出　　处：《中药药理与临床》2024年第11期16-21,共6页Pharmacology and Clinics of Chinese Materia Medica

基　　金：广东省普通高校重点领域专项项目(编号:2023ZDZX2011);广东省普通高校重点领域专项项目(编号:2021ZDZX2015);广州中医药大学“双一流”与高水平大学学科协同创新团队项目;广州中医药大学中医学学科中医基础理论研究“揭榜挂帅”项目。

摘　　要：目的:通过体外实验探讨加味芍药甘草汤对子宫腺肌病(Adenomyosis,AM)异位子宫内膜间质细胞(EScs)的迁移、侵袭和血管生成过程的影响。方法:原代收集、培养并分离EScs,利用细胞免疫组化法进行细胞鉴定;通过Transwell迁移、侵袭试验检测加味芍药甘草汤对EScs迁移、侵袭能力的影响;通过RT-qPCR法检测EScs中血管生成相关因子mRNA的表达;通过免疫荧光标记细胞中微血管密度(MVD)。结果:收集、培养并分离的6例人AM原代细胞均为EScs。与空白对照组相比,1.69 g/kg加味芍药甘草汤10%含药血清组和地诺孕素0.31μg/mL组的细胞迁移和侵袭能力均显著降低(P<0.01),加味芍药甘草汤10%含药血清组Vegf、Hif-1α、Pdgf、Ang、Fgf1和Fgf2的mRNA表达明显下调(P<0.05或P<0.01),MVD免疫荧光强度明显降低(P<0.05),地诺孕素0.31μg/mL组Vegf、Pdgf、Ang和Fgf1的mRNA表达明显下调(P<0.05或P<0.01);与正常对照血清组相比,加味芍药甘草汤10%含药血清组和地诺孕素0.31μg/mL组的细胞迁移和侵袭能力明显降低(P<0.05或P<0.01),加味芍药甘草汤10%含药血清组Fgf1和Fgf2的mRNA表达明显下调(P<0.05),地诺孕素0.31μg/mL组Ang的mRNA表达明显下调(P<0.05)。结论:所获细胞为EScs,加味芍药甘草汤含药血清可以限制EScs的迁移、侵袭能力,抑制血管生成相关因子的表达。Objective:To investigate the effect of modified Shaoyao(芍药)-Gancao(甘草)Decoction on migration,invasion,and angiogenesis of endometrial stromal cells(EScs)in ectopic adenomyosis(AM)through in vitro experiments.Methods:Primary EScs were collected,cultured,and isolated,and cell immunohistochemistry was used to identify EScs.Transwell migration and invasion tests were conducted to detect the effect of modified Shaoyao-Gancao Decoction on the migration and invasion ability of EScs.The mRNA expressions of angiogenesis-related factors in EScs were detected by real-time quantitative polymerase chain reaction(RT-qPCR).Microvascular density(MVD)in the cells was labeled by immunofluorescence.Results:Six human AM primary cells that were collected,cultured,and isolated were all EScs.Compared with those in the control group,the cell migration and invasion ability in the group containing 10% of 1.69 g/kg modified Shaoyao-Gancao Decoction and dienogest group were significantly decreased(P<0.01),the mRNA expressions of Vegf,Hif-1α,Pdgf,Ang,Fgf1,and Fgf2 in the group containing 10% of modified Shaoyao-Gancao Decoction were significantly down-regulated(P<0.05 or P<0.01).Immunofluorescence of MVD was significantly decreased(P<0.05),and mRNA expressions of Vegf,Pdgf,Ang,and Fgf1 in the dienogest group were significantly decreased(P<0.05 or P<0.01).Compared with those in the normal control group,the cell migration and invasion ability in the group containing 10% of modified Shaoyao-Gancao Decoction and dienogest group were significantly decreased(P<0.05 or P<0.01).The mRNA expressions of Fgf1 and Fgf2 in the group containing 10% of modified Shaoyao-Gancao Decoction were significantly down-regulated(P<0.05),and the mRNA expression of Ang in the dienogest group was significantly down-regulated(P<0.05).Conclusion:EScs were collected,and modified Shaoyao-Gancao Decoction could inhibit the migration and invasion of EScs,as well as the expression of angiogenesis-related factors.

关 键 词：加味芍药甘草汤 子宫腺肌病 迁移 侵袭 血管生成 

分 类 号：R73[医药卫生—肿瘤]