铜死亡相关基因COX17在食管癌中的表达及其对Eca109细胞功能的影响  

Differential expression of copper death related gene COX17 in esophageal carcinoma and its effect on Eca109 cell function

作　　者：卢宛瑶 刘伊宁 沙巴海提·吾斯曼 艾乐宇 胡晓文 李静蕾 钟炫宇 艾米热拉·米尔喀迪尔 伊娜 刘玲 LU Wanyao;LIU Yining;SHABAHAITI·Wusiman;AI Leyu;HU Xiaowen;LI Jinglei;ZHONG Xuanyu;AIMIRELA·Mierkadier;YI Na;LIU Ling(Department of Biochemistry and Molecular Biology of Basic Medical Sciences,Xinjiang Medical University,Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases,Urumqi 830017;Department of Clinical Medicine,Xinjiang Medical University,Urumqi 830017;Laboratory Department,The Third People's Hospital of Xinjiang,Urumqi 830091,Xinjiang,China)

机构地区：[1]新疆医科大学基础医学院生物化学与分子生物学教研室,新疆地方病分子生物学重点实验室,新疆乌鲁木齐830017 [2]新疆医科大学临床医学部,新疆乌鲁木齐830017 [3]新疆维吾尔自治区第三人民医院检验科,新疆乌鲁木齐830091

出　　处：《癌变.畸变.突变》2025年第1期45-51,共7页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：新疆医科大学2023年国家级大学生创新训练计划项目(202310760024)。

摘　　要：目的:探讨铜死亡相关基因COX17在食管癌中的表达及其对食管癌Eca109细胞功能的影响。方法:利用UALCAN数据库分析COX17 mRNA表达水平与食管癌患者临床病理特征的相关性。采用免疫组织化学法检测15对食管癌及其对应癌旁组织中COX17蛋白的表达,并进行临床病理资料分析。另采用siRNA干扰敲降食管癌Eca109细胞中COX17的表达,用Western blot检测siR-COX17敲降的效果,并通过CCK-8试验、划痕试验以及Transwell试验检测COX17表达下调时对Eca109细胞增殖、迁移及侵袭能力的影响。结果:UALCAN数据分析显示,与正常对照组比较,COX17 mRNA表达水平在食管癌组织中明显升高,且与肿瘤临床分期、病理分级、性别、年龄、种族具有相关关系(P<0.05)。免疫组织化学法检测结果显示,COX17蛋白在食管癌组织中的表达高于对应癌旁组织,且COX17蛋白的表达水平与病理分级和淋巴结转移相关,差异均具有统计学意义(P<0.05)。siRNA转染后,COX17蛋白在Eca109细胞中的表达水平明显低于对照组,且敲低COX17后抑制了食管癌细胞的增殖、迁移及侵袭能力。结论:铜死亡相关基因COX17在食管癌中高表达并与预后相关,其可能参与了食管癌的生物学恶性进展。OBJECTIVE:To investigate expression of the cuproptosis related gene COX17,in esophageal cancer and its impact on cellular function.METHODS:Correlations between COX17 mRNA and clinical characteristics of esophageal cancer patients were evaluated using the UALCAN database.Immunohistochemistry was used to detect the expression of COX17 in 15 pairs of esophageal cancer and their corresponding adjacent tissues,and clinical pathological data was analyzed.siRNA interference was used to knock down the expression of COX17 in esophageal cancer cell line Eca109.The knockdown effect on siR-COX17 was detected by Western blot,and the effects of COX17 downregulation on the proliferation,migration,and invasion ability of Eca109 cells were detected by CCK-8 assay,scratch assay,and transwell assay.RESULTS:The UALCAN data analysis showed that,compared with the normal control group,expression of COX17 was significantly increased in esophageal cancer,and its expression level was correlated with tumor clinical stage,pathological grading,gender,age,and race(P<0.05).The immunohistochemical detection showed that the expression of COX17 was higher in esophageal cancer tissues than in corresponding adjacent tissues,and the expression level of COX17 was significantly correlated with pathological grading and lymph node metastasis(P<0.05).After siRNA transfection,the expression level of COX17 protein in Eca109 was significantly lower than that in the control group,and the knock down inhibited the proliferation,migration,and invasion ability of esophageal cancer cells.CONCLUSION:The cuproptosis related gene COX17 was highly expressed in esophageal cancer and was associated with prognosis,which mignt be involved in the malignant progression of esophageal cancer.

关 键 词：食管癌 COX17 铜死亡 生物信息学 

分 类 号：R735.1[医药卫生—肿瘤]