不同穴位艾灸对薄型子宫内膜的修复作用研究  

Reparative effects of moxibustion at different acupoints on thin endometrium

作　　者：周海忆 何斯怡 韩瑞芳 关永格 董丽娟[4] 宋阳[1] ZHOU Haiyi;HE Siyi;HAN Ruifang;CUAN Yonge;DONG Lijuan;SONG Yang(School of Nursing,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Lingnan Medical Research Center,Guangzhou University of Chinese Medicine,Guangzhou 510006;the Third Affliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006;Zhongshan Hospital of Traditional Chinese Medicine,Guangdong Province,Zhongshan 528400)

机构地区：[1]广州中医药大学护理学院,广州510006 [2]广州中医药大学岭南医学研究中心,广州510006 [3]广州中医药大学第三附属医院,广州510006 [4]广东省中山市中医院,广东中山528400

出　　处：《中国比较医学杂志》2024年第12期50-58,共9页Chinese Journal of Comparative Medicine

基　　金：广东省自然科学基金面上项目(2024A1515012194);国家中医药传承示范试点项目——中山市中医院中医药科研立项(YN2024B008)。

摘　　要：目的比较艾灸“关元”和“肾俞”穴对薄型子宫内膜大鼠的修复作用。方法将32只雌性SD大鼠随机分为对照(CON)组、模型(MOD)组、关元(GY)组和肾俞(SS)组,使用95%无水乙醇造模处理。关元组和肾俞组分别艾灸“关元”和“肾俞”穴,其余两组相同固定。测量艾灸前后穴区温度变化;HE染色法观察子宫病理形态;ELISA检测血清炎症因子及激素水平;免疫组化法检测增殖和容受性因子表达;Western blot法检测血管生成因子表达。结果艾灸后即刻和5 min后肾俞穴温均高于关元(P<0.01)。与对照组相比,模型组大鼠子宫内膜厚度、腺体及血管数均减少(P<0.01);血清白细胞介素-1β(interleukin 1β,IL-1β)升高(P<0.01),孕酮(progesterone,PROG)降低(P<0.01);子宫中增殖标志物Ki67、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、白血病抑制因子(leukemia inhibitory factor,LIF)、整合素β3(integrinβ3,ITG-β3)表达降低(P<0.01),血管生成素-1(angiopoietin-1,ANG-1)、CD34表达下降(P<0.01)。与模型组相比,关元组和肾俞组内膜厚度、腺体和血管数均增加(P<0.01),IL-1β降低(P<0.05),雌二醇(estradiol,E2)(P<0.05)和PROG增加(P<0.05),肾俞组还提高了白细胞介素-10(interleukin 10,IL-10)水平(P<0.05);两组Ki67、PCNA、LIF、ITG-β3表达增强(P<0.01),关元组PCNA强于肾俞组(P<0.05),肾俞组LIF、ITG-β3强于关元组(P<0.01,P<0.05);两组CD34、VEGFA表达升高(P<0.01),关元组ANG-1高于肾俞组(P<0.01),而肾俞组CD34表达更高(P<0.05)。结论艾灸“关元”和“肾俞”穴均能修复大鼠薄型子宫内膜病理形态,关元组降低大鼠血清IL-1β水平提高E2水平、促进增殖因子表达优于肾俞组,肾俞组提高PROG水平、促进内膜容受性因子表达优于关元组,两穴均能提高血管生成因子的表达,但可能通过不同的途径发挥作用。Objective To compare the reparative effects of moxibustion at“Guanyuan"”and“Shenshu"”points in rats with a thin endometrium.MethodsThirty-two female Sprague-Dawley rats were divided randomly into control(CON),model(MOD),Guanyuan(GY),and Shenshu(SS)groups.A thin endometrium model was established using 95%anhydrous alcohol.The Guanyuan and Shenshu groups underwent moxibustion at the Guanyuan and Shenshu points,respectively,and the other two groups of rats were fixed in the same way.Temperature changes at the acupoint area were measured at different time points.The pathological morphology of the uterus was observed by hematoxylin and eosin(HE)staining,and serum levels of inflammatory factors and hormone levels were detected by enzyme-linked immunosorbent assay(ELISA).Expression levels of proliferation and endometrial receptivity factors were detected by immunohistochemistry(IHC),and angiogenesis factors were detected by Western blot.Results The temperatures immediately after moxibustion and after 5 min were higher at the Shenshu compared with the Guanyuan acupoint(P<0.01).Endometrial thickness and numbers of glands and blood vessels were decreased in the MOD group compared with the CON group(P<0.01),serum interleukin 1β(IL-1β)was increased(P<0.01)and PROG was decreased(P<0.01),and Ki67,proliferating cell nuclear antigen(PCNA),leukemia inhibitory factor(LIF),integrinβ3(ITG-β3),angiopoietin-1(ANG-1),andCD34 protein in uterine tissue were all decreased(P<0.01).Endometrial thickness and numbers of glands and blood vessels were increased in the GY and SS groups compared with the MOD group(P<0.O1),while serum IL-1βwas decreased(P<0.05),E2 and PR0G were increased(P<0.05),and serum interleukin 10(IL-10)was increased in the SS group(P<0.05).Ki67,PCNA,LIF,and ITG-β3 were all increased in both treatment groups compared with the MOD group(P<0.01).PCNA was higher in the GY group compared with the SS group(P<0.05),while LIF and ITG-β3 were higher in the SS group compared with the GY group(P<0.01,P<0.05,respectively

关 键 词：薄型子宫内膜 艾灸 关元穴 肾俞穴 

分 类 号：R-33[医药卫生]