基于特征肽的高效液相色谱-串联质谱法鉴定沙门氏菌  

Identification ofSalmonellaUsing High Performance Liquid Chromatography-Tandem Mass Spectrometry Based on Characteristics Peptides

作　　者：陈梦琪 袁慕云 黄成栋 刘婧文 曾广丰 陈文锐 胡松青[2] 凌莉 CHEN Meng-Qi;YUAN Mu-Yun;HUANG Cheng-Dong;LIU Jing-Wen;ZENG Guang-Feng;CHEN Wen-Rui;HU Song-Qing;LING Li(Guangzhou Customs Technology Center,Guangzhou 510623,China;School of Food Science and Engineering,South China University of Technology,Guangzhou 510641,China)

机构地区：[1]广州海关技术中心,广州510623 [2]华南理工大学食品科学与工程学院,广州510641

出　　处：《分析化学》2024年第12期1913-1922,共10页Chinese Journal of Analytical Chemistry

基　　金：国家重点研发计划项目(No.2022YFF1100805)资助。

摘　　要：通过检测沙门氏菌特征肽,建立了一种基于高效液相色谱-串联质谱(HPLC-MS/MS)多反应监测模式的沙门氏菌定性鉴定方法。针对8条沙门氏菌特征肽,进行了仪器参数优化,确定了各特征肽的最佳离子对和碰撞能量。进一步,评估了方法在菌种鉴定方面的性能。实验结果表明,本方法具有较高的特异性,能够准确区分沙门氏菌与其它常见的食源性致病菌。在牛奶基质中,50%检出限(LOD50)为0.35 CFU/25 g。将此方法应用于5种人工污染样品中的沙门氏菌的定性鉴定,相较传统的生化鉴定方法,本方法具有速度快和特异性高等优点,为食品中沙门氏菌的快速、准确鉴定提供了新方法。A qualitative identification method for Salmonella using high-performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS)with multiple reaction monitoring(MRM)mode was established.Eightcharacteristic peptides were selected,and their MRM parameters were optimized.Furthermore,the performance ofthis HPLC-MS/MS method in species identification was further evaluated.Experimental results demonstrated thatthis method exhibited high specificity,enabling accurate differentiation of Salmonella from conventional foodborne pathogens.In a milk matrix,the 50%limit of detection(LOD50)was determined to be 0.35 CFU/25 g.Thismethod was successfully applied to qualitatively identify Salmonella in five artificially contaminated food samples.The method presented here,in contrast to traditional biochemical assays,offered both speed and specificity,providing a novel means for the rapid and accurate identification of Salmonella in food.It held significantimplications for the surveillance,prevention,and control of food-borne diseases.

关 键 词：沙门氏菌 特征肽 高效液相色谱-串联质谱法 多反应监测 

分 类 号：O657.63[理学—分析化学] TS207.4[理学—化学]