谷氨酸化学交换饱和转移MR成像对脂多糖诱导脓毒症相关性脑病的大鼠血脑屏障损伤的应用研究  

Application research of glutamate chemical exchange saturation transfer MR imaging in the blood-brain barrier injury of rats with sepsis associated encephalopathy induced by lipopolysaccharide

作　　者：钟雅志 吴仁华[2] ZHONG Ya-zhi;WU Ren-hua(Department of Radiology,Huizhou Central People's Hospital,Huizhou Guangdong 516000,China;Department of Medical Imaging,the Second Affiliated Hospital of Shantou University Medical College,Shantou Guangdong 515000,China)

机构地区：[1]惠州市中心人民医院放射科,广东惠州516000 [2]汕头大学医学院第二附属医院医学影像科,广东汕头515000

出　　处：《中国临床医学影像杂志》2025年第1期11-14,共4页Journal of China Clinic Medical Imaging

摘　　要：目的:采用谷氨酸化学交换饱和转移成像(Glu-CEST)观察由脂多糖诱导的脓毒症相关性大鼠脑内的影像改变与大鼠血脑屏障损伤的关系。方法:将24只健康雄性SD大鼠均分为脓毒症相关性脑病(SAE)组(10 mg/kg脂多糖)和对照组(注射与脂多糖等量的生理盐水)两组,并通过旷场实验分别观察两组大鼠行为学表现,其行为学指标包括大鼠的活动总路程、活动时间、站立次数以及跨格次数。采用7.0T小动物专用磁共振扫描仪获得大鼠脑中的Glu-CEST图像。最后通过伊文思蓝实验检测大鼠血脑屏障的损伤。结果:旷场实验的行为学表现表明与对照组对比,SAE组的大鼠的活动时间、站立次数显著下降(P<0.01),提示SAE组大鼠焦虑、抑郁症状更显著;SAE组大鼠脑中的Glu-CEST值((15.20±0.79)%)较对照组((11.93±1.63)%)显著升高(P<0.01),同时SAE组((4.07±0.64)ng/mL)的伊文思蓝溶液渗透结果亦较对照组((1.97±0.23)ng/mL)更显著(P<0.05),且通过Person相关性分析提示伊文思蓝实验的渗透值与Glu-CEST值具有正相关。结论:结合Glu-CEST MRI可在分子影像水平上无创性地反映脂多糖诱导脓毒症相关性脑病的大鼠脑中的血脑屏障受损变化及程度。Objective:To observe the relationship between brain image changes and blood-brain barrier injury in rats with sepsis associated encephalopathy induced by lipopolysaccharide using glutamate chemical exchange saturation transfer(Glu-CEST)imaging.Methods:Twenty-four healthy male SD rats were divided into two groups:the sepsis-associated encephalopathy(SAE)group(10 mg/kg lipopolysaccharide)and the control group(injected with the same amount of normal saline as lipopolysaccharide).Open field experiment was conducted to observe the behavioral performance of the rats in the two groups,including the total distance of activity,activity time,standing times and span times.The image of Glu-CEST in rat brain was obtained by 7.0T MR scanner for small animals.Finally,the damage to the blood-brain barrier in rats was detected by the Evans blue test.Results:Compared with the control group,the activity time and standing times of the SAE group decreased significantly(P<0.01),indicating that anxiety and depression symptoms were more significant in the SAE group.The level of Glu-CEST in the brain of the SAE group((15.20±0.79)%)was significantly higher than that of the control group((11.93±1.63)%)(P<0.01).Meanwhile,the penetration result of Evans blue solution in the SAE group((4.07±0.64)ng/mL)was more significant than that in the control group((1.97±0.23)ng/mL)(P<0.05),and the Person correlation analysis suggested that the infiltration value of Evans blue test was positively correlated with Glu-CEST value.Conclusion:Combination with Glu-CEST MRI can noninvasively reflect the changes and extent of blood-brain barrier damage in rats with lipopolysaccharid-induced SAE at the molecular imaging level.

关 键 词：脓毒症相关性脑病 血脑屏障 磁共振成像 

分 类 号：R742[医药卫生—神经病学与精神病学] R445.2[医药卫生—临床医学]