国产Quick TargSeq全集成自动化现场快速DNA检测仪的测试评估  

Test and Evaluation of the Made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument

作　　者：韩俊萍 王颖希 刘超[3] 刘锋 郭晋荣 朱巍[6] 唐光峰 李彩霞 王鑫 焦章平 HAN Jun-ping;WANG Ying-xi;LIU Chao;LIU Feng;GUO Jin-rong;ZHU Wei;TANG Guang-feng;LI Cai-xia;WANG Xin;JIAO Zhang-ping(Criminal Investigation Detachment,Chaoyang District Branch of Beijing Public Security Bureau,Beijing 100025,China;Forensic Science Service of Beijing Public Security Bureau,Beijing 100192,China;Guangzhou Institute of Criminal Science and Technology,Guangzhou 510030,China;Liaoning Institute of Criminal Science and Technology,Shenyang 110032,China;Science and Technology Division,Department of Public Security of Shanxi Province,Taiyuan 030001,China;Tianjin Institute of Criminal Science and Technology,Tianjin 300384,China;Institute of Criminal Science and Technology,Tai’an Public Security Bureau,Tai’an 271000,Shandong Province,China;Key Laboratory of Forensic Genetics,Beijing Engineering Research Center of Crime Scene Evidence Examination,National Engineering Laboratory for Forensic Science,Institute of Forensic Science of China,Beijing 100038,China;Criminal Investigation Detachment,Huaibei Public Security Bureau,Huaibei 235000,Anhui Province,China)

机构地区：[1]北京市公安局朝阳分局刑事侦查支队,北京100025 [2]北京市公安司法鉴定中心,北京100192 [3]广州市刑事科学技术研究所,广东广州510030 [4]辽宁省刑事科学技术研究所,辽宁沈阳110032 [5]山西省公安厅科技处,山西太原030001 [6]天津市刑事科学技术研究所,天津300384 [7]泰安市公安局刑事科学技术研究所,山东泰安271000 [8]公安部鉴定中心,法医遗传学公安部重点实验室,北京市现场物证检验工程技术研究中心,现场物证溯源技术国家工程实验室,北京100038 [9]淮北市公安局刑事侦查支队,安徽淮北235000

出　　处：《法医学杂志》2024年第5期461-467,共7页Journal of Forensic Medicine

基　　金：北京市现场物证检验工程技术研究中心开放课题资助项目(2021CSEEKFKT06);中央级公益性科研院所基本科研业务费专项(2022JB020,2017JB025)。

摘　　要：目的验证国产Quick TargSeq全集成自动化现场快速DNA检测仪(以下简称“Quick TargSeq快检仪”)的稳定性和准确性,并评估其法医学应用前景。方法采用公安部刑侦局专家组(4名个体共20份)和5家参试单位(54份)提供的合计74份盲样,在5台Quick TargSeq快检仪和微流控芯片卡盒上进行测试,将所得结果与已知分型进行比对,计算样本的检出率和分型准确性。结果专家组提供的20份样本在5台Quick TargSeq快检仪的重复结果均与已知分型一致。74份样本的检出率为100%,分型准确率达97.33%。结论Quick TargSeq快检仪稳定性良好,分型准确性较高。Objective To verify the stability and accuracy of the made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument(hereinafter referred to as Quick TargSeq DNA detection instrument)and evaluate its forensic application prospects.Methods A total of 74 blind samples provided by the expert group of Criminal Investigation Bureau of the Ministry of Public Security(4 individuals,20 samples)and five participating institutions(54 samples)were used to test on 5 Quick TargSeq DNA detection instruments and the accompanying microfluidic chip cartridges.The results were compared with the known genotypes to calculate the samples’detection rate and genotyping accuracy.Results The results of reproducibility obtained from 20 samples provided by the expert group on the 5 Quick TargSeq DNA detection instruments were all consistent with the known genotypes.The detection rate of the 74 samples was 100%,and the genotyping accuracy was 97.33%.Conclusion The Quick TargSeq DNA detection instrument has good stability and high genotyping accuracy.

关 键 词：法医遗传学 法医DNA分型 快速DNA分析 短串联重复序列 族群推断 

分 类 号：R89[医药卫生—法医学] DF795.2[医药卫生—临床医学] D919.2[政治法律—诉讼法学]