醛缩酶A对膀胱癌细胞生物学的影响及机制研究  

Effect of aldolase A on the biology of bladder cancer cells and its mechanism

作　　者：李建伟[1] 蔡芳震[1] 刘昌锦 Li Jianwei;Cai Fangzhen;Liu Changjin(Department of Urology,Second Affiliated Hospital of Fujian Medical University,Quanzhou 360001,China)

机构地区：[1]福建医科大学附属第二医院泌尿外科,泉州360001

出　　处：《中国医药》2025年第2期226-230,共5页China Medicine

基　　金：福建省自然科学基金(2020J01206)。

摘　　要：目的探究醛缩酶A对膀胱癌细胞增殖、迁移、侵袭及上皮-间质转化(EMT)的影响及机制。方法通过慢病毒感染构建干扰或过表达醛缩酶A质粒并转染至T24人膀胱移行细胞癌(T24)细胞中,将细胞分为对照组、空载组、醛缩酶A干扰组和醛缩酶A过表达组。检测醛缩酶A在膀胱癌组织和癌旁组织中的表达。检测细胞增殖、迁移和侵袭情况,同时检测EMT及Wnt/β-连环蛋白信号通路相关蛋白的表达。结果膀胱癌组织中醛缩酶A的免疫组织化学染色评分及相对表达量均高于癌旁组织[(33±7)分比(17±5)分、(63±6)%比(22±4)%](均P<0.001)。细胞计数盒8试验处理24、36和48 h后,醛缩酶A干扰组T24细胞吸光度值均低于空载组,而醛缩酶A过表达组T24细胞吸光度值均高于空载组,差异均有统计学意义(均P<0.05)。醛缩酶A干扰组划痕愈合率低于空载组,醛缩酶A过表达组划痕愈合率高于空载组(均P<0.05)。醛缩酶A干扰组侵袭细胞数少于空载组,醛缩酶A过表达组侵袭细胞数多于空载组(均P<0.05)。醛缩酶A干扰组E-钙黏蛋白相对表达量高于空载组,神经钙黏蛋白、波形蛋白、β-连环蛋白、细胞周期素D1、c-Myc基因蛋白相对表达量均低于空载组(均P<0.05)。醛缩酶A过表达组E-钙黏蛋白相对表达量低于空载组,神经钙黏蛋白、波形蛋白、β-连环蛋白、细胞周期素D1、c-Myc基因蛋白相对表达量均高于空载组(均P<0.05)。结论醛缩酶A可促进膀胱癌细胞增殖、迁移、侵袭和EMT,这可能是通过Wnt/β-连环蛋白信号通路发挥影响的。Objective To investigate the effect and mechanism of aldolase A on the proliferation,migration,invasion and epithelial-mesenchymal transition(EMT)of bladder cancer cells.Methods The interference or overexpression plasmids of aldolase A were constructed by lentivirus infection and transfected into T24 human bladder transitional cell carcinoma(T24)cells.The cells were divided into control group,empty vector group,aldolase A interference group and aldolase A overexpression group.The expression of aldolase A in bladder cancer tissues and adjacent tissues was detected.The cell proliferation,migration and invasion were detected,and the expressions of EMT and Wnt/β-catenin signaling pathway related proteins were detected.Results The immunohistochemical staining score and relative expression of aldolase A in bladder cancer tissues were higher than those in adjacent tissues[(33±7)vs(17±5),(63±6)%vs(22±4)%](both P<0.001).After treatment with Cell Counting Kit-8 assay for 24,36 and 48 h,the absorbance values of T24 cells in aldolase A interference group were lower than those in the empty vector group,while the absorbance values of T24 cells in aldolase A overexpression group were higher than those in the empty vector group(all P<0.05).The scratch healing rate of aldolase A interference group was lower than that of empty vector group,and the scratch healing rate of aldolase A overexpression group was higher than that of empty vector group(both P<0.05).The number of invasive cells in the aldolase A interference group was less than that in the empty vector group,and the number of invasive cells in the aldolase A overexpression group was more than that in the empty vector group(both P<0.05).The relative expression of E-cadherin in the aldolase A interference group was higher than that in the empty vector group,and the relative expression of N-cadherin,vimentin,β-catenin,cyclin D1,and c-Myc gene protein were lower than those in the empty vector group(all P<0.05).The relative expression of E-cadherin in the aldolase A overe

关 键 词：膀胱癌 醛缩酶A 上皮-间质转化 Β-连环蛋白 细胞增殖 

分 类 号：R737.14[医药卫生—肿瘤]