光果甘草内生真菌的分离及其次级代谢产物活性研究  

Isolation of Endophytic Fungi from Glycyrrhiza glabra L.and Study of Their Secondary Metabolite Activity

作　　者：李洋 贾志鹏 赵毓杰 赵肖肖 裴佳梅 陈垲林 冯再平[1] 张新国[1] LI Yang;JIA Zhipeng;ZHAO Yujie;ZHAO Xiaoxiao;PEI Jiamei;CHEN Kailin;FENG Zaiping;ZHANG Xinguo(College of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China)

机构地区：[1]兰州理工大学生命科学与工程学院,兰州730050

出　　处：《世界科学技术-中医药现代化》2024年第11期2996-3005,共10页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会地区科学基金项目(32160021):西部生境中未培养微生物多样性及其重要类群创新抗耐药菌药物的筛选研究,负责人:张新国。

摘　　要：目的从新鲜的光果甘草中分离、纯化内生真菌,并筛选出能够对腺苷脱氨酶、β-内酰胺酶、黄嘌呤氧化酶和酪氨酸酶产生抑制效果的次级代谢产物的菌株。方法采用4种培养基以组织匀浆法分离纯化内生真菌。然后用活性评价平台对其次级代谢产物进行评价。结果分离得到植物内生真菌131株,其中34株形态各异且特殊。其中菌株G(1-5)(s)的菌丝体甲醇提取物有较好的腺苷脱氨酶抑制活性(抑制率:98.58%)和β-内酰胺酶抑制活性(抑制率:93.15%),菌株G6次级代谢产物的乙酸乙酯相有较好的黄嘌呤氧化酶抑制活性(抑制率:91.38%),分别鉴定为Penicillium sp.和Aspergillus sp.。结论光果甘草内生真菌种类丰富,具有良好的研究价值,后期值得深入研究,为光果甘草的保护和深层次应用提供重要的技术资料。Objective Endophytic fungi were isolated and purified from fresh Glycyrrhiza glabra L.and strains capable of producing secondary metabolites with inhibitory effects on adenosine deaminase,β-lactamase,xanthine oxidase,and tyrosinase were screened.Methods Four types of culture media were used to isolate and purify endophytic fungi using tissue homogenization method.Subsequently,the secondary metabolites of endophytic fungi were evaluated using an activity assessment platform.Results A total of 131 strains of endophytic fungi were isolated from the plant,among which 34 strains exhibited distinct morphologies and special characteristics.The mycelial extract of hypha G(1-5)(s)showed good adenosine deaminase inhibitory activity(inhibition rate:98.58%)andβ-lactamase inhibitory activity(inhibition rate:93.15%).andβ-lactamase(inhibition rate:93.15%).Strain G6 exhibited excellent xanthine oxidase inhibitory activity(inhibition rate:91.38%)in the ethyl acetate phase.These strains were identified as Penicillium sp.and Aspergillus sp.Conclusion Glycyrrhiza glabra L.harbors a diverse range of endophytic fungi with promising research potential.Further investigation is warranted to provide important technical data for the protection and in-depth applications of Glycyrrhiza glabra L..

关 键 词：光果甘草 内生真菌 分离 鉴定 次级代谢产物 酶抑制活性 

分 类 号：R284.2[医药卫生—中药学]