磁铁矿对生物膜产量、EPS成分及吸附功能的影响研究  

Study on the effect of magnetite on biofilm yield,EPS composition,and adsorption function

作　　者：邢筱静 沈东升[1] 丁贺宁 龙於洋[1] 惠彩 XING Xiaojing;SHEN Dongsheng;DING Hening;LONG Yuyang;HUI Cai(Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling,Zhejiang Engineering Research Center of Non-ferrous Metal Waste Recycling,School of Environmental Science and Engineering,Zhejiang Gongshang University,Hangzhou 310012)

机构地区：[1]浙江工商大学环境科学与工程学院,浙江省固体废物处理与资源化重点实验室,有色金属废弃物资源化浙江省工程研究中心,杭州310012

出　　处：《环境科学学报》2025年第1期39-49,共11页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.42107006,42177203);浙江工商大学研究生科研创新基金项目(No.YBXM2023090)。

摘　　要：磁铁矿在自然环境中广泛存在,磁铁矿颗粒接触生物膜后会影响其中的胞外聚合物(Extracellular Polymeric Substances,EPS),但不同浓度和尺寸的磁铁矿对EPS分泌量和成分的影响尚未见报道.本文通过在Bacillus amyloliquefaciens DT生物膜形成过程中添加磁铁矿,探究了不同时期添加不同浓度、不同粒径磁铁矿对生物膜产量、形态及EPS分泌量和组分的影响,并研究了生物膜对BDE-47的吸附性能与机制.结果表明,磁铁矿的添加提高了生物膜产量、改变了生物膜形态并促进了溶解态EPS的分泌,最高分泌量达410 mg·L^(-1),其中,添加1 g·L^(-1)50 nm的磁铁矿后产生EPS中蛋白质含量最高.磁铁矿添加时间会影响生物膜产量,菌液静置前添加不同浓度磁铁矿对生物膜影响更大,且小粒径磁铁矿更易促进生物膜的形成.三维荧光光谱和傅里叶红外光谱结果表明,磁铁矿添加刺激了生物膜EPS中类蛋白物质的产生.特征生物膜对BDE-47的吸附实验结果表明,添加1 g·L^(-1)50 nm的磁铁矿后形成的生物膜对BDE-47的吸附效果最好,生物膜中的蛋白质在吸附过程中起主要作用.本研究阐释了磁铁矿对生物膜成分及功能的影响和机制,可为高吸附性能生物膜的制备提供理论基础.Magnetite is widely present in natural environments,and its contact with biofilms can affect the extracellular polymeric substances(EPS)in them.However,the effects of different concentrations and sizes of magnetite on EPS secretion and composition have not been reported.This study investigated the effects of adding varying concentrations and particle sizes of magnetite to the biofilm formation process of Bacillus amyloliquefaciens DT at different time on the biofilm yield,morphology,EPS secretion and composition,as well as its adsorption performance and mechanism for BDE-47.Results indicated that magnetite addition increased biofilm yield,altered biofilm morphology,and promoted the secretion of dissolved EPS,with the highest secretion reaching 410 mg·L^(-1).Notably,the addition of 1 g·L^(-1)50 nm magnetite resulted in the highest protein secretion in EPS.The addition time of magnetite affected the biofilm yield.Adding magnetite with different concentrations before bacterial solution settling had a notable impact on biofilm formation,and magnetite with small particle size was more likely to promote biofilm formation.Results of three-dimensional fluorescence spectroscopy and Fourier-transform infrared spectroscopy indicated that magnetite addition promoted the production of protein-like substances in biofilm.The adsorption experiments of specific biofilms on BDE-47 demonstrated that the addition of 1 g·L^(-1)50 nm magnetite yielded the highest adsorption capacity,and protein in the biofilm played a major role in the adsorption process.This study elucidates the influence and mechanism of magnetite on the composition and function of biofilm,which can provide theoretical basis for the preparation of biofilm with high adsorption properties.

关 键 词：磁铁矿 生物膜 胞外聚合物(EPS) 吸附 

分 类 号：X131[环境科学与工程—环境科学] X172