持续自然混合期水体微生物特性变化及对水质改善影响研究  

Microbiological changes in water bodies during continuous natural mixing and the impact on water quality improvement

作　　者：宋云霞 黄廷林[1,2,3,4] 张轩 寇莉青 张春晓[1,2,3,4] 刘宇轩 蔡晓春 SONG Yunxia;HUANG Tinglin;ZHANG Xuan;KOU Liqing;ZHANG Chunxiao;LIU Yuxuan;CAI Xiaochun(Key Laboratory of Northwest Water Resource,Environment and Ecology,Ministry of Education,Schoolof Environmental and Municipal Engineering,Xi'an University of Architecture and Technology,Xi'an 710055;Shaanxi Key Laboratory of Environmental Engineering,Schoolof Environmental and Municipal Engineering,Xi'an University of Architecture and Technology,Xi'an 710055;Collaborative Innovation Center of Water Pollution Control and Water Quality Security Assurance of Shaanxi Province,Xi'an University of Architecture and Technology,Xi'an 710055;Shaanxi Provincial Field Scientific Observation and Research Station of Water Quality in Qinling Mountains,Xi'an University of Architecture and Technology,Xi'an 710055;Xi'an Water Supply Corporation(Group)Lijiahe Reservoir Management Co.Ltd,Xi'an 710016)

机构地区：[1]西安建筑科技大学环境与市政工程学院,西北水资源与环境生态教育部重点实验室,西安710055 [2]西安建筑科技大学环境与市政工程学院,陕西省环境工程重点实验室,西安710055 [3]西安建筑科技大学,陕西省水污染控制与水质安全保障协同创新中心,西安710055 [4]西安建筑科技大学,秦岭水源地水质陕西省野外科学观测研究站,西安710055 [5]西安水务(集团)李家河水库管理有限公司,西安710016

出　　处：《环境科学学报》2025年第1期216-227,共12页Acta Scientiae Circumstantiae

基　　金：国家重点研发计划课题(No.2022YFC3203604);陕西省重点研发计划项目(No.2022ZDLSF06-08)。

摘　　要：近年来,大中型水库逐渐成为城市主要的饮用水来源,其水质安全逐渐成为全球关注的问题.原位实验证明,扬水曝气系统可以破坏水库水体分层,人为诱导水体提前进入自然混合时期,比未经人为干预的水体提前了2.5个月.然而,对于持续自然混合期的水体生物特性(碳代谢活性、群落结构及多样性)变化与水质改善之间的关系仍缺乏深入研究.为此,本文系统研究了持续自然混合期李家河水库水质参数、污染物浓度、细菌碳代谢活性、物种组成和微生物群落共生模式的时空变化.持续自然混合初期水体菌群具有较高的丰度及碳代谢活性,随着持续混合的进行,均呈现逐渐下降的趋势.整个持续自然混合期水体细菌群落结构以放线菌门和变形菌门为主,优势属为CL500-29_marine_group(26.65%)和hgcI_clade(18.21%).共生网络分析表明持续自然混合初期细菌群落间相互作用更复杂紧密.冗余分析则表明微生物种群结构受环境因子和水质因子的共同调控.相关性分析表明功能微生物与水质间具有显著相关性,对水质改善起到积极作用.总体而言,该研究结果将为饮用水水源水质改善原位生物修复提供科学依据.In recent years,large and medium-sized reservoirs have gradually become the main source of drinking water for cities,raising concerns about water safety,particularly regarding its quality.In situ experiments have demonstrated that the Water-lifting and aeration systems(WLAs)can disrupt the stratification of reservoir water bodies and artificially induce water bodies to enter the natural mixing period 2.5 months earlier than those without human intervention.Nevertheless,there is a paucity of comprehensive research investigating the relationship between alterations in biological characteristics(carbon metabolism activity,community structure and diversity)and the enhancement of water quality in water bodies during the sustained natural mixing period.To address this issue,this study comprehensively investigated the spatial and temporal changes in water quality parameters,pollutant concentrations,bacterial carbon metabolic activity,species composition and microbial community symbiosis patterns in Lijiahe Reservoir during the sustained natural mixing period.At the onset of the sustained natural mixing period,the water column's bacterial community exhibited high abundance and carbon metabolism activity,both of which demonstrated a declining trend as the sustained mixing progressed.Throught the entire period of continuous natural mixing,the bacterial community structure in the water body was dominated by Actinobacteria and Ascomycetes,with the dominant genera being CL500-29_marine_group(26.65%)and hgcI_clade(18.21%).Symbiotic network analyses indicated that the interactions among bacterial communities were more complex and tightly knit during the early stages of sustained natural mixing.In contrast,redundancy analyses demonstrated that microbial population structure was co-regulated by environmental and water quality factors.Correlation analysis demonstrated a significant relationship between the functional microorganisms and water quality,with microorganisms exhibiting a positive influence on water quality improvement.T

关 键 词：饮用水水库 持续自然混合 生物改善 共生网络分析 污染物去除 

分 类 号：TU991.2[建筑科学—市政工程] X172[环境科学与工程—环境科学]