茶树GASA基因家族的生物信息学分析  

Bioinformatics analysis of GASA gene family in tea tree

作　　者：王艳 叶凡 王坤 聂嘉玲 吴媛媛 刘国峰 刘慧娟 WANG Yan;YE Fan;WANG Kun;NIE Jialing;WU Yuanyuan;LIU Guofeng;LIU Huijuan(College of Life Sciences/Henan Key Laboratory of Tea Plant Biology,Xinyang Normal University,Xinyang 464000,China;College of Forestry and Biotechnology,Zhejiang Agriculture and Forestry University,Hangzhou 310000,China;College of Science/Tropical Biodiversity and Bioresource Utilization Laboratory,Qiongtai Normal University,Haikou 571127,China)

机构地区：[1]信阳师范大学生命科学学院/河南省茶树生物学重点实验室,河南信阳464000 [2]浙江农林大学林业与生物技术学院,浙江杭州310000 [3]琼台师范学院理学院/热带生物多样性与资源利用实验室,海南海口571127

出　　处：《信阳师范大学学报(自然科学版)》2025年第1期24-33,共10页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(81700283);信阳师范大学“南湖学者奖励计划”青年项目。

摘　　要：GASA(Gibberellic acid-stimulated in arabidopsis)是植物特有的转录因子家族,与植物的生长、发育、抗逆密切相关。采用生物信息学方法,基于茶树的全基因组序列,共鉴定到了29个GASA基因,根据染色体排列,编号为CsGASA 1—CsGASA 29。系统发育分析、保守基序分析、共线性分析、启动子元件分析及表达分析显示:CsGASA分为3个亚家族,编码64~218个氨基酸;共鉴定到6个保守基序;有4个CsGASA来源于片段重复事件,5个CsGASA来源于串联重复事件;有53%的启动子元件与环境响应有关;有部分CsGASA基因受到干旱、低温和盐胁迫调控;CsGASA 3基因只在根处具有较高的表达量。GASA(Gibberellic acid-stimulated in arabidopsis)is a group of plant-specific transcription factors related to plant growth,development and stress response.Using a bioinformatics approach to explore the whole genome sequence of tea tree,29 GASA genes were identified in the tea tree genome and numbered according to the chromosomal arrangement as CsGASA 1-CsGASA 29.Phylogenetic analysis,conserved motif analysis,collinearity analysis,cis-acting element analysis and expression analysis were performed to show that CsGASA is classified into three subfamilies and encodes 64-218 amino acids;a total of six conserved motifs are identified;four CsGASA are originated from segmental duplication events,and five CsGASA from tandem duplication events;53%of the cis-acting elements are the key links in plant environmental response;some of CsGASA gene expression are response to drought,low temperature and salt stresses,and CsGASA 3 gene is highly expressed only in roots.

关 键 词：茶树 GASA基因 进化分析 生物信息学分析 

分 类 号：S571.1[农业科学—茶叶生产加工]