基于生物信息学分析三阴性乳腺癌对药物治疗反应的关键差异表达基因  

Identification of Gene Changes Induced by Drug Therapy in the Triple Negative Breast Cancer Using Bioinformatics Analysis

作　　者：霍丹丹 张军慧 邱建阁 刘文静 王萌 李军涛 赵二江 HUO Dandan;ZHANG Junhui;QIU Jiange;LIU Wenjing;WANG Meng;LI Juntao;ZHAO Erjiang(The Affiliated Cancer Hospital of Zhengzhou University&Henan Cancer Hospital,Zhengzhou 450008,China;Heart Center of Henan Province People's Hospital/Fuwai Central China Cardiovascular Hospital/Fuwai Central China Hospital of Zhengzhou University,Henan Key Lab for Prevention and Control of Coronary Heart Disease/Henan Institute of Cardiovascular Epidemiology,Zhengzhou 451464,China;the Academy of Medical Science,Zhengzhou University,Zhengzhou 450003,China)

机构地区：[1]郑州大学附属肿瘤医院/河南省肿瘤医院,河南郑州450008 [2]河南省人民医院心脏中心/华中阜外医院/郑州大学华中阜外医院,河南省冠心病防治重点实验室/河南省心血管流行病学研究中心,河南郑州451464 [3]郑州大学医学科学院,河南郑州450003

出　　处：《河南医学研究》2025年第2期193-197,共5页Henan Medical Research

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20200192,LHGJ20190783);河南省科技攻关项目(242102310063)。

摘　　要：目的基于生物信息学方法,从分子层面研究三阴性乳腺癌(TNBC)细胞使用多西他赛化疗反应的差异表达基因(DEGs),为研究三阴性乳腺癌诊疗提供新的思路。方法从美国国立生物技术信息中心(NCBI)的公共基因芯片数据库(GEO)中获取TNBC基因芯片数据集GSE70690,应用计算机编程语言(R语言)软件筛选DEGs,并利用DAVID在线数据库对该基因数据进行相关基因功能富集分析。应用String在线数据库构建蛋白-蛋白相互作用网络(PPI),筛选出多西他赛化疗反应的关键基因。结果分析芯片数据,筛选出865个差异表达基因,其中上调差异基因395个,下调差异基因470个。基因本体(GO)功能分析及京都基因与基因组百科全书(KEGG)通路分析结果显示,差异表达基因与DNA复制、细胞分裂有关,并富集在DNA复制信号通路上。通过构建PPI网络筛选得到了CDK1、TOP2A和BRCA1是三阴性乳腺癌患者多西他赛化疗分子机制中的枢纽基因。结论利用生物信息学方法筛选出TNBC化疗药物多西他赛3个枢纽基因和关键信号通路,为提升TNBC化疗效果提供新的理论依据。Objective To study the differentially expressed genes(DEGs)of triple negative breast cancer(TNBC)cells in response to chemotherapy with docetaxel based on bioinformatics methods,and to provide new ideas for the diagnosis and treatment of TNBC.Methods The TNBC gene-on-chip dataset GSE70690 was obtained from the public Gene Expression Omnibus(GEO)of the National Center for Biotechnology Information(NCBI)in the United States:The DEGs was screened using R language software,and the gene function enrichment analysis was performed using the DAVID online database.The protein-protein interaction networks(PPI)was constructed using String online database to screen out the key genes of docetaxel chemotherapy response.Results A total of 865 differentially expressed genes were selected by microarray data analysis,including 395 up-regulated differential genes and 470 down-regulated differential genes.The results of gene ontology(GO)function analysis and Kyoto Encyclopedia of Genes and Genomes(KECGG)pathway analysis showed that differentially expressed genes were related to DNA replication and cell division,and were concentrated in the DNA replication signaling pathway.Through the construction of PPI network screening,CDKI,TOP2A and BRCAI were the key genes in the molecular mechanism of docetaxel chemotherapy in triple negative breast cancer patients.Conclusion Three key genes and key signaling pathways of Docetaxel for TNBC chemotherapy were screened by bioinformatics method,providing a new theoretical basis for improving the efficacy of TNBC chemotherapy.

关 键 词：三阴性乳腺癌 生物信息学 差异表达基因 

分 类 号：R737.9[医药卫生—肿瘤]