降低牛心中嘌呤含量的工艺研究  

Optimization of the process to reducing purine content in cattle heart

作　　者：陈玲 苏伟 吴文清 李子涵 母应春 焦鱼 CHEN Ling;SU Wei;WU Wenqing;LI Zihan;MU Yingchun;JIAO Yu(School of Brewing and Food Engineering,Guizhou University,Guiyang 550025)

机构地区：[1]贵州大学酿酒与食品工程学院,贵阳550025

出　　处：《中国食品添加剂》2025年第2期99-105,共7页China Food Additives

基　　金：国家自然科学基金地区项目(32160554);贵州省科技厅科技服务项目(黔科合服企[2020]4009(006));贵州科技厅科技重大专项(黔科合重大专项字[2020]3009号)。

摘　　要：利用核酸酶降低牛心中嘌呤有利于牛心资源的开发利用,采用高效液相色谱法(High Performance Liquid Chromatography,HPLC)测定牛心中嘌呤物质的含量,以单因素试验和正交试验确定核酸酶P1处理牛心的最佳工艺条件。结果表明,牛心内所含的四种嘌呤化合物腺嘌呤、鸟嘌呤、次黄嘌呤和黄嘌呤的含量分别为235.2±0.5、166.7±1.2、381.2±1.3、127.9±1.8 mg/kg,总嘌呤含量为911.0±1.4 mg/kg。酶添加量(A)、处理时间(B)和处理温度(C)对牛心中嘌呤的降解有显著的影响,影响因素主次排序为A>C>B;工艺参数最优组合为A_(2)B_(2)C_(3),即每0.2 mg牛心样品的酶添加量为24 U/mL、处理时间为1 h、温度为70℃,在此处理条件下牛心内的嘌呤降解率为55%。此研究采用核酸酶P1处理能显著降低牛心中嘌呤物质的含量,可为低嘌呤牛心产品开发提供理论基础。The use of nucleases to reduce purine levels in cattle heart contributes to the development and utilization of cattle heart resources.The content of purine substance in cattle heart was determined by high performance liquid chromatography(HPLC),and the optimal process conditions for nuclease P1 treatment were determined by single factor tests and orthogonal tests.The results showed that the content of four purine compounds were as follows:adenine 235.2±0.5,guanine content 166.7±1.2,hypoxanthine content 381.2±1.3,and xanthine content 127.9±1.8 mg/kg,with a total purine content of 911.0±1.4 mg/kg.The amount of enzyme added(A),processing time(B)and processing temperature(C)were found to have significant influence on the degradation of purines in cattle heart,with the order of influencing factors being A>C>B;the optimal combination of process parameters was identified as A_(2)B_(2)C_(3),corresponding to an enzyme addition of 24 U/mL per 0.2 mg of cattle heart sample,a processing time of 1 h and a temperature of 70℃,resulting in a cattle heart purine degradation of 55%under this processing conditions.This study demonstrated that treatment with nuclease P1 could significantly reduce the content of purine substance in the cattle heart,providing a theoretical basis for the development of low-purine cattle heart products.

关 键 词：牛心 嘌呤 高效液相色谱法(HPLC) 核酸酶 

分 类 号：TS202.3[轻工技术与工程—食品科学]