AcCdr4对炭黑曲霉生长和赭曲霉毒素A合成的调控功能  

Regulated Function of AcCdr4 on Growth and Ochratoxin A Production of Aspergillus carbonarius

作　　者：姜楠[1,2] 王刘庆 姜冬梅 姚卫蓉 王蒙[1] JIANG Nan;WANG Liuqing;JIANG Dongmei;YAO Weirong;WANG Meng(Institute of Quality Standard and Testing Technology of Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097;School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122)

机构地区：[1]北京市农林科学院质量标准与检测技术研究所,北京100097 [2]江南大学食品学院,江苏无锡214122

出　　处：《核农学报》2025年第3期437-448,共12页Journal of Nuclear Agricultural Sciences

基　　金：北京市自然科学基金面上项目(6222012);北京市农林科学院科技创新能力建设专项(KJCX20240336)。

摘　　要：AcCdr4是一种氨基酸序列具有多处跨膜域的ABC转运蛋白,该蛋白可能影响炭黑曲霉(Aspergillus carbonarius)的生长及赭曲霉毒素A(OTA)的产生。为了明确ABC转运蛋白AcCdr4的调控功能,本研究构建了炭黑曲霉AcCdr4基因敲除突变株,对该基因参与调控炭黑曲霉生长和OTA合成的功能进行了初步探索。结果表明,AcCdr4基因敲除后,炭黑曲霉在体外培养基和葡萄果实上生长发育缓慢,OTA产量显著下降。进一步对ΔAcCdr4突变株中OTA合成基因簇基因的表达量进行分析,发现Acpks、Acnrps、AcbZIP的相对表达量显著下调,菌体对OTA的分泌能力下降,说明该基因参与调控炭黑曲霉中OTA毒素的合成。此外,以丁香酚作为抗真菌剂处理AcCdr4敲除突变株,发现AcCdr4基因敲除后菌体对丁香酚的敏感性增强,说明该基因有助于菌体抵抗外源活性物质的刺激。本研究为探明炭黑曲霉生长发育及产毒的分子机制提供了理论数据,为研制新型抗真菌药物提供了潜在靶标。AcCdr4 is an ABC transporter with multiple transmembrane domains,which may affect the growth and production of Ochratoxin A(OTA)in Aspergillus carbonarius.In order to clarify the regulatory role of AcCdr4,a AcCdr4 knockout mutant of A.carbonarius was created,and the function of this gene in regulating both growth and OTA synthesis of A.carbonarius was preliminarily investigated.The results showed that knocking out the AcCdr4 gene resulted in slower growth and development of A.carbonarius in in vitro culture medium and on grape fruits,and the production of OTA was significantly reduced.Further analysis of the expression levels of genes within the OTA synthesis gene cluster revealed that the relative expression levels of Acpks,Acnrps and AcbZIP inΔAcCdr4 mutant were significantly down-regulated.The secretion ability of OTA decreased,indicating that AcCdr4plays a role in regulating the synthesis of OTA in A.carbonarius.Additionally,treating AcCdr4 knockout mutant strains with eugenol,an antifungal agent,increased the sensitivity of the strain to eugenol,indicating that this gene contributes to the resistance against the stimulation of exogenous active substances for A.carbonarius.This study provides theoretical insights into the molecular mechanisms underlying the growth,development,and OTA production of A.carbonarius,and identifies potential targets for the development of new antifungal drugs.

关 键 词：炭黑曲霉 OTA ABC转运蛋白 AcCdr4 调控功能 

分 类 号：TS201.6[轻工技术与工程—食品科学]