Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid analysis of eight Gelsemium elegans Benth alkaloids in human plasma and urine  

作　　者：Xi Zeng Yu Wang Lin Luo Yina Lu Zhenlin Xu 

机构地区：[1]Guangdong Provincial Key Laboratory of Food Quality and Safety,South China Agricultural University,Guangzhou 510642,China [2]Guangzhou Institute of Food Inspection,Guangzhou 511400,China [3]Shantou Customs District,Shantou 515041,China

出　　处：《One Health Advances》2024年第1期20-30,共11页全健康进展(英文)

基　　金：supported by the National Natural Science Foundation of China(Grant No.32372448);Guangdong Basic and Applied Basic Research Foundation,China(Grant No.2023A1515012605);the Science and Technology Program of Guangdong Administration for Market Regulation,China(Grant No.2023CS01);the Science and Technology Program of National General Customs Administration of China(Grant No.2022HK108);the Science and Technology Program of Shantou City,China(Grant No.STKJ2023024).

摘　　要：Gelsemium elegans Benth alkaloids are the main components of G.elegans and can cause acute toxicosis or even death.Although several studies have reported methods for detecting G.elegans alkaloids,a high-throughput and environmental-friendly strategy for detection of multiple G.elegans alkaloids has not been realized.In this work,a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method was developed for rapid detection of G.elegans alkaloids in human plasma and urine for diagnosis of poisoning.Multiple matrices and crys-tal spotting methods were evaluated to obtain stable and high peak intensities without“sweet spot”.We verified the methodology and obtained excellent results.The matrix effects with different dilutions were compared and good recoveries and a low relative standard deviation were obtained with a 40-fold dilution.This method could shorten the analysis time and greatly reduce the consumption of chemical solvents.Furthermore,it could be applied to quan-titative assessment of G.elegans alkaloid poisoning incidents.

关 键 词：Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Gelsemium elegans Benth High-throughput trial ALKALOID Acute poisoning Biological sample 

分 类 号：O62[理学—有机化学]