Development of a lateral flow immunochromatography assay for the detection of fluoroacetamide in blood samples  

作　　者：Qiang Li Ling Yang Changfei Duan Xiaonan Wang Xuezhi Yu 

机构地区：[1]National Key Laboratory of Veterinary Public Health and Safety,Beijing Key Laboratory of Detection Technology for Animal Derived Food Safety,Beijing Laboratory for Food Quality and Safety,College of Veterinary Medicine,China Agricultural University,Beijing 100193,People’s Republic of China [2]Department of Food and Bioengineering,Beijing Vocational College of Agriculture,Beijing 102442,People’s Republic of China

出　　处：《One Health Advances》2024年第1期289-297,共9页全健康进展(英文)

基　　金：supported by Beijing Municipal Science and Technology Commission(Z211100007021007);the earmarked fund for CARS36,the Key R&D Program of Ningxia Hui Autonomous Region(2021BBF02036);the program of Beijing Municipal Education Commission(KM202212448002).

摘　　要：Fluoroacetamide(FAM)has been employed as a rodenticide for an extended duration,leading to a multitude of inci-dents involving human ingestion poisoning.Currently,FAMs have been prohibited by nations globally;however,there are still instances of their illegal usage.Conventional instrument methods are characterized by their time-consuming nature and complex operational procedures,rendering them inadequate for meeting urgent diagnostic needs in patients with acute FAM poisoning.Therefore,there is an immediate need to develop a prompt,user-friendly,and precise immunoassay method for the diagnosis of acute poisoning induced by FAM.A lateral flow immunochro-matography assay(LFIA)was developed in this study for the visual detection of FAMs in blood samples,representing the first report of such an approach.The method exhibited a cut-off value of 0.5 mg/mL under the optimized condi-tions,enabling the entire FAM detection process in blood samples to be completed within a mere 8 min without any pretreatment requirements.Notably,the results were easily discernible by visual inspection alone.These results indi-cate that the developed LFIA holds great promise as a convenient and rapid diagnostic tool for FAM poisoning diag-nosis,thereby offering valuable support for subsequent treatment strategies.

关 键 词：FLUOROACETAMIDE RODENTICIDE Lateral flow immunochromatography assay Blood samples 

分 类 号：R73[医药卫生—肿瘤]