A patch comprising human umbilical cord-derived hydrogel and mesenchymal stem cells promotes pressure ulcer wound healing  

作　　者：Liqin Chen Ying Zhang Kexin Wang Meixian Jin Qi Chen Simin Wang Wei Hu Zhai Cai Yang Li Shao Li Yi Gao Shuqin Zhou Qing Peng 

机构地区：[1]Department of Anesthesiology,The Second Affiliated Hospital,School of Medicine,The Chinese University of Hong Kong,Shenzhen&Longgang District People’s Hospital of Shenzhen,Shenzhen,518172,China [2]Department of Anesthesiology,Zhujiang Hospital,Southern Medical University,Guangzhou,510000,China [3]Department of central laboratory,The Second Affiliated Hospital,School of Medicine,The Chinese University of Hong Kong,Shenzhen&Longgang District People’s Hospital of Shenzhen,Shenzhen,518172,China [4]General Surgery Center,Department of Hepatobiliary Surgery II,Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering,Guangzhou Clinical Research and Transformation Center for Artificial Liver,Institute of Regenerative Medicine,Zhujiang Hospital,Southern Medical University,Guangzhou,510000,China [5]Department of Anesthesiology,The First Affiliated Hospital of Jinan University,Guangzhou,510630,China [6]Department of General Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou,510000,China

出　　处：《Engineered Regeneration》2024年第4期433-442,共10页再生工程(英文)

基　　金：Funded by the Guangdong Basic and Applied Basic Research Foundation(2023A1515012452,2021B1515230011);the Science and Technology Program of Guangzhou(2024B03J0022,201803010086)。

摘　　要：Pressure ulcers(PUs)are common skin injuries known for their high morbidity,rapid onset,susceptibility to infection,and challenging healing process.One potential therapy for PUs is cell-based therapy using mesenchymal stem cells(MSCs).However,poor survival and low cell retention of MSCs on skin lesions limit their therapeutic effects and applications.In this study,we prepared an extracellular matrix(dECM)hydrogel decellularized from the human umbilical cord(UC).A patch composed of UC-dECM and UC-MSCs was employed in the treatment of PUs in C57BL/6 mice.Our results indicate that the UC-dECM hydrogel effectively sustains cell viability,enhances the stemness-related gene expression in UC-MSCs,and promotes human umbilical vein endothelial cells(HUVECs)migration and angiogenesis.Compared to the groups treated with the patch containing only UC-dECM,injection of UC-MSCs or gauze dressing,the patch combining UC-dECM hydrogel with UC-MSCs significantly accelerated PU healing.This positive outcome can be attributed to the promotion of tissue re-epithelialization,collagen deposition,angiogenesis,and inflammation inhibition.Our results suggest that the composite patch,comprised of UC-dECM hydrogel and UC-MSCs,may be a promising therapeutic approach for PU treatment.

关 键 词：Pressure ulcers Human umbilical cord mesenchymal stem cells Decellularized extracellular matrix Patch Tissue regeneration 

分 类 号：R73[医药卫生—肿瘤]