Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder  

作　　者：Shiwen Liu Xuerui Wan Meng Zhao Jiaqi Wang Weilan Wu Linlin You Yonggui Yuan Qian Xu Rong Gao 

机构地区：[1]Key Laboratory of Environmental Medicine Engineering,Ministry of Education,School of Public Health,Southeast University,Nanjing 210009,China [2]Key Laboratory of Modern Toxicology of Ministry of Education,Department of Hygienic Analysis and Detection,School of Public Health,Nanjing Medical University,Nanjing 211166,China [3]Department of Psychosomatics and Psychiatry,Zhongda Hospital,School of Medicine,Southeast University,Nanjing 210009,China

出　　处：《Science China Chemistry》2025年第1期377-384,共8页中国科学(化学英文版)

基　　金：supported by the National Natural Science Foundation of China(82273684)。

摘　　要：The measurement of amino acid and monoamine neurotransmitters(NTs)in serum plays a central role in the identification and monitoring of clinical panic disorder(PD).However,the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely challenging.Herein,we developed a strategy that integrated solid phase extraction based on magnetic Fe_(3)O_(4)coated with polydopamine(Fe_(3)O_(4)@PDA)and direct analysis of mass spectrometry in real time(DART-MS)for quantification of 10 NTs.We systematically investigated the optimal conditions of this strategy to achieve good accuracy and precision.Compared with existing magnetic solid phase extraction methods for NTs detection in biological samples,this strategy could adsorb more targets while having similar adsorption efficiencies,and the total process time was reduced by an average of 69.42%.We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic accuracy.In addition,an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this strategy.Our results highlighted the potential ability of Fe_(3)O_(4)@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

关 键 词：NEUROTRANSMITTERS panic disorder magnetic solid phase extraction direct analysis of mass spectrometry in real time 

分 类 号：R749[医药卫生—神经病学与精神病学] O657.63[医药卫生—临床医学]