Modulation of liver metabolism and gut microbiota by Alhagi-honey alleviated heat stress-induced liver damage  

作　　者：Jing Xu Yundie Liu Xuanhong Cao Xinrui Guo Jie Wang Yang Liu Hongda Zhou Baohua Ma Sha Peng 

机构地区：[1]College of Veterinary Medicine,Northwest A&F University,Yangling 712100,Shaanxi,China

出　　处：《Stress Biology》2024年第1期174-186,共13页逆境生物学(英文)

基　　金：supported by the Natural Sciences Foundation of China[32072815];Grants from the National Key Research and Development Program of China[2022YFD1300200];the General Project of the Key R&D Plan of Shaanxi Province[2023-YBNY-140];Horizontal Research Funding from Ningbo Second Hormone Factory[TG20221184].

摘　　要：Alhagi-honey(AH)is a well-established traditional ethnic medicine with advantageous effects against diarrhea and headaches.We aimed to explore the preventive effect of AH on liver damage induced by heat stress(HS)and its underlying mechanism.HS models were established by thermostat,and mice were treated at 39℃for 10 h,lasting for 7 days.Hematoxylin-eosin(H&E)staining and Periodic Acid-Schiff(PAS)staining were used for histological observation,and transmission electron microscopy(TEM)was used for ultrastructure examination of hepatocytes.Gut microbiota(GM)composition and liver metabolites were respectively analyzed by 16S rRNA sequencing and nontargeted metabolome sequencing.AH pretreatment alleviated liver damage caused by heat stress in mice.The main manifestation was that AH alleviated serum aspartate transferase(AST)and aspartate transaminase(ALT).It was found that AH improved symptoms of hepatocyte damage.In addition,the relative abundance of f_Rikenellaceae,g_Incertae_Sedis and s_Staphylococcus_Orisratti,g_Lachnoclostridium,g_GCA-900066575,and s_Alistipes_inops were modified by AH and these bacterial genera showed association with 6 metabolites(2-(3,4-dihydroxyphenyl)acetamide,3-hydroxy-3-methylpentanedioic acid,PC(17:0/17:1),Y-L-Glutamy-L-glutamic acid,L-Isoleucine,5-Methyluridine,8,8-dimethyl-2-phenyl-4H,8H-pyrano[2,3-h]chromen-4-one).The Pearson analysis also showed a strong correlation between these microbes and 2 risk indicators(AST and ALT)of liver damage.AH alleviated HS-induced liver damage by regulating liver metabolism and maintaining normal GM.It demonstrated that AH held potential as a prophylactic drug for the prevention of HS-induced liver damage.

关 键 词：Alhagi-honey Gut microbiota Liver damage Heat stress 

分 类 号：R57[医药卫生—消化系统]