Time-restricted feeding relieves high temperature-induced impairment on meat quality by activating the Nrf2/HO-1 pathway,modification of muscle fiber composition,and enriching the polyunsaturated fatty acids in pigs  

作　　者：Zhaojian Li Yiting Wang Peng Yuan Yanli Zhu Ping Hu Tongxing Song Rui Liu Hao-Yu Liu Demin Cai 

机构地区：[1]Laboratory of Animal Physiology and Molecular Nutrition,Jiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design,College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China [2]College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Stress Biology》2024年第1期205-220,共16页逆境生物学(英文)

基　　金：supported by the Natural Science Foundation of Jiangsu Province(BK20220582);Jiangsu Funding Program for Excellent Postdoctoral Talent,the Jiangsu Agricultural Science And Technology Innovation Fund[CX(21)2014];Jiangsu Provincial Double-Innovation Team Program(JSSCTD202147);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：To assess the effects of a time-restricted feeding(TRF)regimen on meat quality of pigs exposed to high ambient temperature,a two-month feeding and heat treatment(HT)trial was conducted using a 2×2 factorial design.A total of 24 growing pigs(11.0±1.9 kg)were randomly divided into four groups:thermal neutral group(NT,24±3°C),HT group(exposed to a high temperature at 35±2°C from 11:00 to 15:00),TRF group and HT+TRF group(HT and TRF co-treatment group,n=6 for each group).Pigs in TRF groups got access to feed within 5 h from 9:00 to14:00,while the others were fed at 6:00,11:30,and 16:00.All pigs received the same diet during the trail.The results showed that HT increased the drip loss,shear force,lightness,and malondialdehyde production in Longissimus thoracis et lumborum(LTL)muscle.TRF reversely reduced the shear force and drip loss,accompanied by decreased intramuscular fat and increased moisture content.Enhanced fiber transformation from type 1 to type 2b and down-regulated expression of muscle growth-related genes were observed by HT,while TRF suppressed the fiber transformation and expression of muscle atrophy-related genes.Furthermore,TRF restored the diminished protein expressions of Nrf2 and HO-1 in LTL muscle by chronic HT.Accumulation of HSP70 in muscle of HT group was reduced by treatment of TRF.HT declined the expression of vital genes involved in fatty acids poly-desaturation and the proportion of(polyunsaturated fatty acids)PUFAs,mainly omega-6 in LTL muscle,while TRF group promoted the expression of poly-desaturation pathway and displayed the highest proportion of PUFAs.These results demonstrated that TRF relieved the chronic high temperature affected meat quality by the restored expression of Nrf2/HO-1 anti-oxidative cascade,modified muscle fiber composition,and enriched PUFAs in LTL muscle.

关 键 词：Meat quality Time-restricted feeding High ambient temperature Muscle fiber composition NRF2 Fatty acids composition 

分 类 号：R73[医药卫生—肿瘤]