RT-PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis  

作　　者：Ying Li Ruiqi Xiao Peicen Zou Yue Du Qinglin Lu Jun Cui Yajuan Wang 

机构地区：[1]Department of Neonatology,Children's Hospital,Capital Institute of Pediatrics,Beijing,China [2]Capital Institute of Pediatrics,Beijing,China [3]Children's Hospital Capital Institute of Pediatrics,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing,China

出　　处：《iLABMED》2024年第4期307-315,共9页智能检验医学(英文)

基　　金：Special Fund of the Pediatric Medical Coordinated Development Center of Beijing Municipal Administration of Hospitals,Grant/Award Number:XTYB201806;the National Natural Science Foundation of China,Grant/Award Number:81872676;Foundation of Capital Institute of Pediatrics,Grant/Award Number:LCPY‐2021‐01。

摘　　要：Background:Early identification of pathogenic bacteria and monitoring re-sidual status are essential for accurate treatment of neonatal bacterial menin-gitis(NBM).Methods:Clinical data and specimens were collected from neonates with NBM.Bacterial cultures and RT-PCR of blood and cerebrospinal fluid(CSF)were compared to assess the positivity rate,sensitivity and specificity of each method.Results:RT-PCR had a higher positivity rate compared with cultures,regardless of whether antibiotics had been used prior to specimen collection.After 1 week of regular antibiotic treatment,the number of pathogen DNA copy numbers in CSF was either undetectable or significantly reduced compared with previous levels.Conclusions:RT-PCR is expected to provide a basis for the precise application of antibiotics and the course of treatment for NBM,particularly in patients with negative cultures or those who have already been treated with antibiotics.

关 键 词：bacterial meningitis CULTURE NEONATE PATHOGEN RT‐PCR 

分 类 号：R73[医药卫生—肿瘤]