Enhancing tissue regeneration studies with luciferase-transgenic rats and bioluminescence imaging:A comprehensive review  

作　　者：Poh-Ching Tan Eiji Kobayashi Qingfeng Li Shuangbai Zhou 

机构地区：[1]Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China [2]Department of Kidney Regenerative Medicine,Jikei University School of Medicine,Tokyo 105-8461,Japan

出　　处：《Chinese Journal of Plastic and Reconstructive Surgery》2024年第4期214-218,共5页中国整形与重建外科(英文)

基　　金：supported by grants from the National Natural Science Foundation of China(grant no.82272287);the Shanghai Clinical Research Center of Plastic and Reconstructive Surgery supported by the Science and Technology Commission of Shanghai Municipality(grant no.22MC1940300);the Cross-Disciplinary Research Fund of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(grant no.JYJC202215);the“National Double First-Class”and“Shanghai Top-Level”high education initiative at Shanghai Jiao Tong University School of Medicine and Shanghai Key Research Center-Shanghai Research Center for Plastic Surgery(grant no.2023ZZ02023).

摘　　要：Bioluminescence imaging(BLI)in rodent models has revolutionized preclinical research in recent decades,enabling precise and noninvasive observation of cellular and molecular processes in vivo.Among various bioluminescent systems,the firefly luciferase-luciferin system is one of the most widely employed for in vivo cell tracking.This comprehensive review focuses on using luciferase-transgenic(Luc-Tg)rat models,known as firefly rats,in conjunction with BLI to investigate tissue regeneration and stem cell dynamics.Compared with other imaging modalities,BLI offers enhanced tissue penetration,reduced background noise,and the capacity to perform longitudinal studies with fewer animals,aligning with ethical research principles.Applications of Luc-Tg rats in fat grafting,soft tissue expansion,hair growth cycle analysis,and other skin studies are discussed,demonstrating the versatility and precision of BLI in tracking complex biological processes.Integrating advanced analytical and genome-editing techniques with BLI promises to enhance data interpretation’s accuracy and efficiency.These advancements have deepened our understanding of the cellular fate and mechanisms underlying tissue regeneration,presenting promising avenues for optimizing therapeutic strategies in reconstructive surgery and regenerative medicine.Combining luciferase reporter genes and BLI is crucial to unraveling complex biological phenomena,advancing soft tissue regeneration research,and developing innovative therapeutic strategies for various medical conditions.

关 键 词：Firefly rat Fat grafting LUCIFERASE Stem cells Skin expansion 

分 类 号：F41[经济管理—产业经济]